二氢硫辛酰胺脱氢酶

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二氢硫辛酰胺脱氢酶

PDB渲染的1zy8
有效结构
PDB 直系同源检索:PDBe, RCSB
标识
代号 DLD; DLDH; E3; GCSL; LAD; PHE3
扩展标识 遗传学238331 鼠基因107450 同源基因84 GeneCards: DLD Gene
EC編號 1.8.1.4
直系同源体
物种 人类 鼠类
Entrez 1738 13382
Ensembl ENSG00000091140 ENSMUSG00000020664
UniProt P09622 O08749
mRNA序列 NM_000108 NM_007861.4
蛋白序列 NP_000099 NP_031887.2
基因位置 Chr 7:
107.53 – 107.57 Mb
Chr 12:
32.02 – 32.04 Mb
PubMed查询 [1] [2]
二氢硫辛酰胺脱氢酶
识别码
EC编号 1.8.1.4
CAS号 9001-18-7
数据库
IntEnz IntEnz浏览
BRENDA BRENDA入口
ExPASy NiceZyme浏览
KEGG KEGG入口
MetaCyc 代谢路径
PRIAM 概述
PDB RCSB PDB PDBe PDBsum
基因本体 AmiGO / EGO

二氢硫辛酰胺脱氢酶EC 1.8.1.4英语Dihydrolipoamide dehydrogenase,缩写DLD,又称为线粒体二氢硫辛酸脱氢酶dihydrolipoyl dehydrogenase, mitochondrial)是一种由人类基因DLD[1][2][3][4]所编码的黄素蛋白,其作用是将二氢硫辛酰胺脱氢从而转化为氧化型硫辛酰胺

DLD作为一种线粒体蛋白质,在真核生物的能量代谢中起到重要作用,它至少参与了五种多酶复合体,且为复合体完成反应所必需的组份[5]。另外,DLD作为一种黄素蛋白氧化還原酶,以FAD为辅基接受质子与电子催化二硫键的形成。

DLD是大小为51千道尔顿亚基的同二聚体,其中每个亚基都与一分子的FAD以共价键的形式相连[6]

功能[编辑]

哺乳动物线粒体三羧酸循环中,该酶与丙酮酸脱氢酶二氢硫辛酰基乙酰基转基酶一起组成中丙酮酸脱氢酶复合体,同时该酶亦是α-酮戊二酸脱氢酶复合体中的一个组分。二氢硫辛酰胺脱氢酶的主要任务是负责将复合物中另一组分硫辛酰胺转乙酰基酶(或二氢硫辛酰琥珀酰转移酶)的辅基二氢硫辛酰胺脱氢从而转化为氧化型硫辛酰胺,该酶也是线粒体甘氨酸剪切体系的组分。

参考文献[编辑]

  1. ^ Entrez Gene: dihydrolipoamide dehydrogenase. 
  2. ^ Otulakowski G, Robinson BH. Isolation and sequence determination of cDNA clones for porcine and human lipoamide dehydrogenase. Homology to other disulfide oxidoreductases. J. Biol. Chem. December 1987, 262 (36): 17313–8. PMID 3693355. 
  3. ^ Pons G, Raefsky-Estrin C, Carothers DJ, Pepin RA, Javed AA, Jesse BW, Ganapathi MK, Samols D, Patel MS. Cloning and cDNA sequence of the dihydrolipoamide dehydrogenase component human alpha-ketoacid dehydrogenase complexes. Proc. Natl. Acad. Sci. U.S.A. March 1988, 85 (5): 1422–6. doi:10.1073/pnas.85.5.1422. PMC 279783. PMID 3278312. 
  4. ^ Scherer SW, Otulakowski G, Robinson BH, Tsui LC. Localization of the human dihydrolipoamide dehydrogenase gene (DLD) to 7q31----q32. Cytogenet. Cell Genet. 1991, 56 (3-4): 176–7. doi:10.1159/000133081. PMID 2055113. 
  5. ^ Babady NE, Pang YP, Elpeleg O, Isaya G. Cryptic proteolytic activity of dihydrolipoamide dehydrogenase. Proceedings of the National Academy of Sciences of the United States of America. 2007, 104 (15): 6158–63. doi:10.1073/pnas.0610618104. PMC 1851069. PMID 17404228. 
  6. ^ Ciszak EM, Makal A, Hong YS, Vettaikkorumakankauv AK, Korotchkina LG, Patel MS. How dihydrolipoamide dehydrogenase-binding protein binds dihydrolipoamide dehydrogenase in the human pyruvate dehydrogenase complex. The Journal of Biological Chemistry. 2006, 281 (1): 648–55. doi:10.1074/jbc.M507850200. PMID 16263718. 

深入阅读[编辑]

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  • Brown AM, Gordon D, Lee H, et al.. Association of the dihydrolipoamide dehydrogenase gene with Alzheimer's disease in an Ashkenazi Jewish population.. Am. J. Med. Genet. B Neuropsychiatr. Genet. 2004, 131B (1): 60–6. doi:10.1002/ajmg.b.30008. PMID 15389771. 
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  • Wang YC, Wang ST, Li C, et al.. The role of N286 and D320 in the reaction mechanism of human dihydrolipoamide dehydrogenase (E3) center domain.. J. Biomed. Sci. 2007, 14 (2): 203–10. doi:10.1007/s11373-006-9136-0. PMID 17171578. 
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外部链接[编辑]

二氢硫辛酰胺脱氢酶引用了美国国家医学图书馆提供的資料,这些資料属于公共领域