SK-BR-3:修订间差异

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2020年1月5日 (日) 07:59的版本

SK-BR-3是在1970年由紀念斯隆-凱特琳癌症中心的科研人員分離的人類乳癌細胞系,目前應用於乳癌治療方案的研究,尤其是在靶向HER2/neu的研究[1]

特徵

HER2/neu產物在SkBr3細胞中過表達

SK-BR-3細胞與AU565細胞分離自同一名曾接受過放射及5-氟尿嘧啶等治療的患者[2]。SK-BR-3細胞源自於一名43歲白人女性患者體內,由乳癌引起的胸腔積液英语Pleural effusion[1],並且過度表達着HER2/neu產物,而該產物被認為與多種乳癌增殖的途徑有關。SK-BR-3細胞已知會以葡萄狀簇 (grape-like clusters)的形式生長,其侵襲性表型與體內細胞類似[3]

科研用途

SK-BR-3細胞已用於尋求能克服曲妥珠單抗對乳腺癌 (過度表達人類表皮生長因子受體2)的抵抗力的研究[4]。而且有科研人員已經檢查了該細胞系在CRISPR/Cas9基因編輯與轉染中的抗體抗性[5],以及在微環境存在波動的情況下,基於HER2/neu的癌症治療中的應用[6]

參考資料

  1. ^ 1.0 1.1 SK-BR-3: Human Breast Cancer Cell Line (ATCC HTB-30). Memorial Sloan-Kettering Cancer Center. [2018-06-18]. 
  2. ^ SK-BR-3 cells. Addex Bio. [2018-06-18]. 
  3. ^ Holliday, DL; Speirs, V. Choosing the right cell line for breast cancer research.. Breast cancer research : BCR. 2011-08-12, 13 (4): 215 [2020-01-05]. PMID 21884641. doi:10.1186/bcr2889. 
  4. ^ Tseng, PH; Wang, YC; Weng, SC; Weng, JR; Chen, CS; Brueggemeier, RW; Shapiro, CL; Chen, CY; Dunn, SE; Pollak, M; Chen, CS. Overcoming trastuzumab resistance in HER2-overexpressing breast cancer cells by using a novel celecoxib-derived phosphoinositide-dependent kinase-1 inhibitor.. Molecular pharmacology. 2006-11, 70 (5): 1534–41 [2020-01-05]. PMID 16887935. doi:10.1124/mol.106.023911. 
  5. ^ Cao, J; Wu, L; Zhang, SM; Lu, M; Cheung, WK; Cai, W; Gale, M; Xu, Q; Yan, Q. An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting.. Nucleic acids research. 2016-11-02, 44 (19): e149 [2020-01-05]. PMID 27458201. doi:10.1093/nar/gkw660. 
  6. ^ Weigelt, B; Lo, AT; Park, CC; Gray, JW; Bissell, MJ. HER2 signaling pathway activation and response of breast cancer cells to HER2-targeting agents is dependent strongly on the 3D microenvironment.. Breast cancer research and treatment. 2010-07, 122 (1): 35–43 [2020-01-05]. PMID 19701706. doi:10.1007/s10549-009-0502-2. 

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