# 离心

## 數學公式

${\displaystyle RPM={\sqrt {g \over r}}}$,

${\displaystyle RCF=1.118\times 10^{-5}\times r\times (rpm)^{2}}$,

## 歷史

1900年時，大家已普遍接受蛋白質是由胺基酸所組成。但有關蛋白質是高分子還是膠體，當時還有爭議[11]。當時在研究的蛋白質是血红蛋白，已知有712個碳原子、1,130個氫原子、243個氧原子、2個硫原子，和至少1個鐵原子。因此红蛋白的重量約是16,000原子质量单位（Da），但還不確定此數值是否要乘以4（表示一個血红蛋白中有四個鐵原子）[12]

## 參考資料

1. ^ Centrifugation Theory. Fischer Scientific. Thermo Fisher Scientific. [2018-03-09]. （原始内容存档于2019-08-20）.
2. ^ Frei, Mark. Centrifugation Basics. Sigma-Aldrich. [2016-05-10]. （原始内容存档于2021-04-30）.
3. ^ Centrifugation. Lenntech. [2013-10-15]. （原始内容存档于2021-07-12）.
4. ^ Garrett, Reginald H.; Grisham, Charles M. Biochemistry 5th. Belmont, CA: Brooks/Cole, Cengage Learning. 2013: 111. ISBN 9781133106296.
5. ^ Zielinski, Sarah. What Is Enriched Uranium?. Smithsonian Magazine. [2020-11-22]. （原始内容存档于2022-04-12）.
6. Ballou, David P.; Benore, Marilee; Ninfa, Alexander J. Fundamental laboratory approaches for biochemistry and biotechnology 2nd. Hoboken, N.J.: Wiley. 2008: 43. ISBN 9780470087664.
7. Burtis, Carl A.; Ashwood, Edward R.; Bruns, David E. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics - E-Book. Elsevier Health Sciences. 2012-10-14 [2021-07-11]. ISBN 978-1-4557-5942-2. （原始内容存档于2021-07-11） （英语）.
8. ^ NÜVE | Centrifugation Tips. www.nuve.com.tr. [2021-07-11]. （原始内容存档于2021-07-11）.
9. Van Holde, K. E. (1998). Analytical ultracentrifugation from 1924 to the present: A remarkable history. Chemtracts – Biochemistry and Molecular Biology. 11:933-943
10. Svedberg, T. (1927). The Ultracentrifuge Nobel Lecture
11. Tanford, C., and Reynolds, J. 2001. Nature’s robots: A history of proteins. Oxford University Press. pp. 303-305
12. ^ Simoni, D. S., Hill, R. L., and Vaughan, M. (2002). The structure and function of hemoglobin: Gilbery Smithson Adair and the Adair equations. The Journal of Biological Chemistry. 277(31): e1-e2
13. ^ Brakke, Myron K. Density Gradient Centrifugation: A New Separation Technique. J. Am. Chem. Soc. April 1951, 73 (4): 1847–1848. doi:10.1021/ja01148a508.
14. ^ Oster, Gerald; Yamamoto, Masahide. Density Gradient Techniques. Chem. Rev. June 1963, 63 (3): 257–268. doi:10.1021/cr60223a003.

## 來源

• Harrison, Roger G., Todd, Paul, Rudge, Scott R., Petrides D.P. Bioseparations Science and Engineering. Oxford University Press, 2003.
• Dishon, M., Weiss, G.H., Yphantis, D.A. Numerical Solutions of the Lamm Equation. I. Numerical Procedure. Biopolymers, Vol. 4, 1966. pp. 449–455.
• Cao, W., Demeler B. Modeling Analytical Ultracentrifugation Experiments with an Adaptive Space-Time Finite Element Solution for Multicomponent Reacting Systems. Biophysical Journal, Vol. 95, 2008. pp. 54–65.
• Howlett, G.J., Minton, A.P., Rivas, G. Analytical Ultracentrifugation for the Study of Protein Association and Assembly. Current Opinion in Chemical Biology, Vol. 10, 2006. pp. 430–436.
• Dam, J., Velikovsky, C.A., Mariuzza R.A., et al. Sedimentation Velocity Analysis of Heterogeneous Protein-Protein Interactions: Lamm Equation Modeling and Sedimentation Coefficient Distributions c(s). Biophysical Journal, Vol. 89, 2005. pp. 619–634.
• Berkowitz, S.A., Philo, J.S. Monitoring the Homogeneity of Adenovirus Preparations (a Gene Therapy Delivery System) Using Analytical Ultracentrifugation. Analytical Biochemistry, Vol. 362, 2007. pp. 16–37.