A-375
A-375 (亦稱ATCC CRL-1619) 是人類惡性黑色素瘤細胞系，最初分離自一名54歲的女性惡性黑色素瘤患者[1]，並且有研究指出A-375細胞對Sutherlandia frutescens敏感[2]。該細胞系在經抗胸腺細胞球蛋白及血清處理的NIH瑞士小鼠中產生生長速度迅速的、類似黑色素瘤的皮下腫瘤。 同時應用於溶瘤肽LTX-315的溶瘤活性研究。它是具有62個染色體的二倍體細胞，每個細胞普遍存在着9個標記染色體，而且每個細胞以一個拷貝存在着正常的N2、N6和N22[1]。有研究指出抑制多種細胞增殖的新狼毒素A以時間及劑量依賴性的方式顯著抑制A375細胞的生長和增殖，細胞體積變小且變得皺縮，核質濃縮且形成凋亡小體[3]。
參考資料[编辑]
- ^ 1.0 1.1 Giard, DJ; Aaronson, SA; Todaro, GJ; Arnstein, P; Kersey, JH; Dosik, H; Parks, WP. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.. Journal of the National Cancer Institute. 1973-11, 51 (5): 1417–23 [2019-12-06]. PMID 4357758. doi:10.1093/jnci/51.5.1417.
- ^ van der Walt, NB; Zakeri, Z; Cronjé, MJ. The Induction of Apoptosis in A375 Malignant Melanoma Cells by Sutherlandia frutescens.. Evidence-based complementary and alternative medicine : eCAM. 2016, 2016: 4921067 [2019-12-06]. PMID 27656236. doi:10.1155/2016/4921067.
- ^ Zhang, Chong; Zhou, Shuang-shuang; Feng, Lin-yi; Zhang, Da-yong; Lin, Neng-ming; Zhang, Li-huang; Pan, Jian-ping; Wang, Jun-bo; Li, Jie. In vitro anti-cancer activity of chamaejasmenin B and neochamaejasmin C isolated from the root of Stellera chamaejasme L. Acta Pharmacologica Sinica. 2012-12-10, 34 (2): 262–270 [2019-12-06]. doi:10.1038/aps.2012.158.