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Deaminase
Introduction
[编辑]Deaminases are a crucial class of enzymes that play a significant metabolic and regulatory role in organisms. They catalyse the deamination reaction of amino acid molecules, removing amino groups and generating corresponding ketone acids. This process is an essential step in the amino acid metabolism pathway, which converts amino acids into other biomolecules, such as energy, proteins, or other metabolites.[1][2][3][4][5][6][7][8].Deaminases are present in various organisms and tissues, and have multiple subtypes and isomers[9][10][11][12][13][14][15] . These enzymes have different specificities and functions, adapting to different biological metabolic needs. In many organisms, deaminases play a crucial role in amino acid metabolism, degradation, and synthesis pathways[16][17][18][19].Researchs have shown that deaminases have broad application prospects in fields such as medicine, industrial production, and agriculture[20][21][22][23][24][25]. A deep understanding of the structure and function of deaminases can lead to the development of more effective drugs, biocatalysts, and industrial production methods, offering greater potential for human health and production activities.
History
[编辑]The development of deaminases can be traced back to the late 19th and early 20th centuries. In 1899, German scientist Ernst Abrams discovered an enzyme in his experiment that could catalyze the deamination of amino acids[26] .However, a deeper understanding of the structure and function of deaminases was not gained until the mid-20th century. During the 1950s and 1960s, scientists conducted extensive research on the biochemical properties of deaminases and identified their crucial role in amino acid metabolism pathways[27][28]. Multiple subtypes and isomers of deaminases were discovered during this period, and their expression in various organisms and tissues was thoroughly investigated. During the 1980s and 1990s, the study of genomics and genetic engineering of deaminases began with the development of molecular biology and biotechnology[29][30]. Scientists can now produce these enzymes on a large scale and conduct more in-depth research on their structure and function by cloning and expressing deaminase genes. In the 21st century, a comprehensive analysis of the deaminase family is underway due to the rapid development of bioinformatics and computational biology[31][32][33][34][35][36] .Genomics and proteomics techniques enable scientists to gain a comprehensive understanding of the distribution, structure, and function of deaminases in living organisms. Deaminases have important applications in fields such as medicine, agriculture, and industry, as demonstrated by numerous studies. This promotes further in-depth development of deaminase research.
Classify
[编辑]Deaminases can be classified into different categories based on their mechanisms of action, substrate specificity, structure, and biological functions.
1.Classification based on substrate specificity
[编辑](1) General deaminases: This deaminase has a broad substrate specificity and can act on various amino acids. For instance, it includes aspartate deaminases and alanine deaminases[37]. (2) Specific deaminase: This deaminase type exhibits high selectivity for specific amino acids. For instance, glutamate deaminase acts specifically on glutamate, and arginine deaminase acts specifically on arginine. This specificity plays a crucial regulatory role in certain metabolic pathways[38].
2.Classification based on structure
[编辑](1) α- Amino acid deaminase: This type of deaminase contains one or more α-amino acid groups in its active site, which catalyse deamination by reacting with substrate amino acids[39]. Examples of typical representatives include aspartate deaminase and alanine deaminase. (2) β- Amino acid deaminase: This type of deaminase contains one or more β-amino acid groups at its active site. Through interaction with substrate amino acids, the β-carbon undergoes a reaction to catalyze deamination[40]. Glutamate deaminase serves as a typical example of a β-amino acid deaminase.
3.Classification based on mechanism of action
[编辑](1) Non oxidizing deaminase: This deaminase type does not require oxygen to participate in the reaction. It mainly completes the substrate deamination reaction through the action of deaminase enzymes. (2) Oxidative deaminase: This type of deaminase requires oxygen as a cofactor to complete the deamination reaction by oxidizing the amino groups of the substrate. Typical representatives include arginine deaminase[41].
4.Properties
[编辑](1) Optimal temperature:
Deaminases typically exhibit optimal activity at moderate temperatures, usually ranging from 30°C to 40°C, depending on the living environment and physiological adaptation of the source organism. However, deaminases from extreme environmental organisms, such as microorganisms in hot springs or organisms in extremely cold regions, may have higher or lower optimal temperatures, respectively[42]. For instance, certain heat source deaminases may display optimal activity above 50°C, whereas some deaminases in cold environments may exhibit optimal activity below 10°C.
(2) Optimal pH:
The optimal pH value for deaminases varies depending on the chemical environment of the source organism and active site. Typically, the optimal pH for most deaminases is neutral to weakly alkaline, around 7.0 to 8.5, which is consistent with the pH of many metabolic pathways within the organism. However, there are exceptions where the optimal pH for some deaminases may be acidic or alkaline. The variation can be explained by the distinct functional needs and adaptive environments of deaminases across various organisms[43].
(3) Substrate specificity:
Deaminases typically exhibit high selectivity for specific types of amino acids based on the structure and conformation of their active sites. For instance, aspartate deaminase specifically targets aspartic acid, and glutamate deaminase specifically targets glutamate. However, some deaminases have broad substrate specificity and can act on various types of amino acids.
(4) Catalytic mechanism:
Deaminases typically use covalent bonding between specific residues in the active site and substrate amino acids to detach and transfer the amino groups of the substrate molecules to the catalytic site. Enzyme-catalysed proton transfer or redox reactions are usually involved in this process. The specific catalytic mechanism may vary depending on the type of deaminase. Experimental research and structural analysis are required for further analysis[44][45].
Synthesis steps of deaminases
[编辑]The synthesis steps of deaminases typically include the following key steps:
1.Gene cloning and sequence analysis
[编辑]First, it is necessary to clone the deaminase gene from the source organism and perform sequence analysis to confirm its integrity and accuracy. This step typically involves operations such as PCR amplification, DNA purification, restriction endonuclease digestion and vector ligation.
2.Construction of expression vector
[编辑]Inserting the deaminase gene into a suitable expression vector typically involves selecting a vector that can efficiently express it in host cells, such as a plasmid or viral vector. In addition, appropriate promoters, activators and selective marker genes must be added to ensure efficient expression of the deaminase gene in host cells[46][47][48].
3.Transfection or transformation of host cells
[编辑]Transfect or transform the constructed expression vector into the target host cell, making it a tool for expressing deaminases[49].
4.Protein expression and purification
[编辑]By optimising the cultivation conditions and protein purification process, high purity deaminase proteins have been obtained. This step typically includes cell culture, protein expression induction, cell fragmentation, protein purification and other operations.
5.Activity detection and functional verification
[编辑]Perform activity testing and functional validation on the purified deaminase protein to confirm its expected enzyme activity and substrate specificity.
6.Applied research and industrial production
[编辑]The r.esulting deaminase protein will be used in research and applications in areas such as medicine, industrial production and agriculture, for example as a catalyst for drug synthesis and a regulator of amino acid degradation.
Applications
[编辑]Deaminases have a wide range of applications in medicine, industry and agriculture: In the pharmaceutical field, deaminases can be used to synthesise pharmaceutical intermediates such as antibiotics, hormones and anticancer drugs. They are also used as biocatalysts for the synthesis of chiral compounds, improving synthesis efficiency and selectivity[50]. In industry, deaminases are widely used in biocatalytic synthesis, organic synthesis and enzyme engineering[51]. For example, they can be used in the production of synthetic spices, food additives and polymers. In agriculture, deaminases can be used in the development of genetically modified crops to improve their nitrogen use efficiency and increase crop yield. They can also be used in the development of biological herbicides and agricultural wastewater treatment[52].
Optimum storage temperature
[编辑]The optimal storage temperature for deaminase is usually between -20°C and -80°C. Within this temperature range, deaminases can be stored stably for months or even years without inactivation. Frozen storage protects the structure and activity of the protein[53][54].
Biological toxicity and hazard potential
[编辑]As an enzyme protein, deaminase normally has no direct biological toxicity. However, if deaminase is ingested in large quantities or if it is exposed to inappropriate environments for a long time after entering the human body, it may cause adverse or allergic reactions[55]. In addition, deaminases may form waste or pollutants in industrial production and appropriate treatment and protection measures must be taken[56]
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