蚓激酶：修订间差异

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2019年2月22日 (五) 01:43的版本

蚓激酶（Lumbrokinase）是一種纖溶酶^[1]^{[註 1]}，大小介於25-32kDa之間^[2]，可以多種途徑促進纖維蛋白的溶解，除了具有纖溶酶活性外，還可作為纖維蛋白溶酶原激活物以刺激纖溶酶的生成，又可促進組織間其他纖維蛋白溶酶原激活物的活性^[3]。蚓激酶最早於1991年在正蚓屬（英语：Lumbricus）Lumbricus bimastus體內發現^[2]^[4]，亦存在於赤子愛勝蚓、粉正蚓（英语：Lumbricus rubellus）與等多種蚯蚓的體腔與消化道內，這種纖溶酶結構穩定，且在人體中可被小腸吸收，因而有作為抗血栓（英语：antithrombotic）藥物的潛力^[5]，且不同於鏈激酶等其他種專一性較低的抗血酸藥物，蚓激酶對纖維蛋白的專一性很高，只有纖維蛋白存在時才具有酵素活性，而較無造成纖溶亢進（英语：hyperfibrinolysis）導致大出血（英语：hemorrhage）之虞^[2]^[6]。蚓激酶已在臨床上被用於治療中風與其他心血管疾病^[2]。

註釋

^ 一種促進纖維蛋白凝塊分解的蛋白質，能產生纖溶作用（英语：fibrinolytic）。

參考資料

^ Hu, R. Codon optimization, expression, and characterization of recombinant lumbrokinase in goat milk. Protein Expression and Purification. 2004, 37 (1): 83–8. PMID 15294284. doi:10.1016/j.pep.2004.05.018.
^ ^2.0 ^2.1 ^2.2 ^2.3 Wang YH, Chen KM, Chiu PS, Lai SC, Su HH, Jan MS, Lin CW, Lu DY, Fu YT, Liao JM, Chang JT, Huang SS. Lumbrokinase attenuates myocardial ischemia-reperfusion injury by inhibiting TLR4 signaling. Journal of Molecular and Cellular Cardiology. 2016, 99: 113-122. doi:10.1016/j.yjmcc.2016.08.004.
^ GangQiang Li, Kevin Yueju Wang, DaHui Li, Nan Wang, DeHu Liu. Cloning, Expression and Characterization of a Gene from Earthworm Eisenia fetida Encoding a Blood-Clot Dissolving Protein. PLoS One: e53110. doi:10.1371/journal.pone.0053110.
^ Hisashi MIHARA, Hiroyuki SUMI, Tomoyuki YONETA, Hideaki MIZUMOTO, Ryuzo IKEDA, Masao SEIKI, Masugi MARUYAMA. A Novel Fibrinolytic Enzyme Extracted from the Earthworm, Lumbricus rubellus. The Japanese Journal of Physiology: 461-472. doi:10.2170/jjphysiol.41.461.
^ Ge, T.; Sun, Z.; Fu, S.; Liang, G. Cloning of thrombolytic enzyme (lumbrokinase) from earthworm and its expression in the yeast. Protein Expression and Purification. 2005, 42 (1): 20–8. PMID 15927482. doi:10.1016/j.pep.2005.04.005.
^ Edwin L.Cooper, M.Balamurugan. Unearthing a source of medicinal molecules. Drug Discovery Today. 2010, 15 (21-22): 966-972. doi:10.1016/j.drudis.2010.09.004.

这是一篇與生物化學相關的小作品。你可以通过编辑或修订扩充其内容。

[2] 一種促進纖維蛋白凝塊分解的蛋白質，能產生纖溶作用（英语：fibrinolytic）。

[1] Hu, R. Codon optimization, expression, and characterization of recombinant lumbrokinase in goat milk. Protein Expression and Purification. 2004, 37 (1): 83–8. PMID 15294284. doi:10.1016/j.pep.2004.05.018.

[Wang2016-3] 2.0 ^2.1 ^2.2 ^2.3 Wang YH, Chen KM, Chiu PS, Lai SC, Su HH, Jan MS, Lin CW, Lu DY, Fu YT, Liao JM, Chang JT, Huang SS. Lumbrokinase attenuates myocardial ischemia-reperfusion injury by inhibiting TLR4 signaling. Journal of Molecular and Cellular Cardiology. 2016, 99: 113-122. doi:10.1016/j.yjmcc.2016.08.004.

[4] GangQiang Li, Kevin Yueju Wang, DaHui Li, Nan Wang, DeHu Liu. Cloning, Expression and Characterization of a Gene from Earthworm Eisenia fetida Encoding a Blood-Clot Dissolving Protein. PLoS One: e53110. doi:10.1371/journal.pone.0053110.

[5] Hisashi MIHARA, Hiroyuki SUMI, Tomoyuki YONETA, Hideaki MIZUMOTO, Ryuzo IKEDA, Masao SEIKI, Masugi MARUYAMA. A Novel Fibrinolytic Enzyme Extracted from the Earthworm, Lumbricus rubellus. The Japanese Journal of Physiology: 461-472. doi:10.2170/jjphysiol.41.461.

[6] Ge, T.; Sun, Z.; Fu, S.; Liang, G. Cloning of thrombolytic enzyme (lumbrokinase) from earthworm and its expression in the yeast. Protein Expression and Purification. 2005, 42 (1): 20–8. PMID 15927482. doi:10.1016/j.pep.2005.04.005.

[7] Edwin L.Cooper, M.Balamurugan. Unearthing a source of medicinal molecules. Drug Discovery Today. 2010, 15 (21-22): 966-972. doi:10.1016/j.drudis.2010.09.004.

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@@ 第2行： / 第2行： @@
 '''蚓激酶'''（Lumbrokinase）是一種[[纖溶酶]]<ref>{{Cite journal|last1=Hu|first1=R.|title=Codon optimization, expression, and characterization of recombinant lumbrokinase in goat milk|journal=Protein Expression and Purification|volume=37|issue=1|pages=83–8|year=2004|doi=10.1016/j.pep.2004.05.018|pmid=15294284
-}}</ref><ref group="註">一種促進[[纖維蛋白]][[血栓|凝塊]]、[[血浆蛋白|血漿蛋白]]分解的蛋白質，能產生{{link-en|纖溶作用|fibrinolytic}}。</ref>，大小介於25-32[[原子质量单位|kDa]]之間，由[[纖維蛋白溶酶原激活物]]與[[纖溶酶]]組成<Ref name=Wang2016>{{Cite journal|title=Lumbrokinase attenuates myocardial ischemia-reperfusion injury by inhibiting TLR4 signaling|author=Wang YH, Chen KM, Chiu PS, Lai SC, Su HH, Jan MS, Lin CW, Lu DY, Fu YT, Liao JM, Chang JT, Huang SS|journal=''Journal of Molecular and Cellular Cardiology''|volume=99|page=113-122|doi=10.1016/j.yjmcc.2016.08.004|year=2016}}</ref>，最早於1991年在{{link-en|正蚓屬|Lumbricus}}''[[Lumbricus bimastus]]''體內發現<Ref name=Wang2016/><Ref>{{cite journal|title=A Novel Fibrinolytic Enzyme Extracted from the Earthworm, Lumbricus rubellus|author=Hisashi MIHARA, Hiroyuki SUMI, Tomoyuki YONETA, Hideaki MIZUMOTO, Ryuzo IKEDA, Masao SEIKI, Masugi MARUYAMA|journal=''The Japanese Journal of Physiology''|volume=41|issue=3|doi=10.2170/jjphysiol.41.461|page=461-472}}</ref>，亦存在於{{link-en|赤子愛勝蚓|Eisenia fetida}}、{{link-en|粉正蚓|Lumbricus rubellus}}與等多種[[蚯蚓]]的[[體腔]]與[[消化道]]內，這種纖溶酶結構穩定，且在人體中可被[[小腸]]吸收，因而有作為{{link-en|抗血栓|antithrombotic}}藥物的潛力<ref>{{Cite journal| last1 = Ge | first1 = T.| last2 = Sun | first2 = Z.| last3 = Fu | first3 = S.| last4 = Liang | first4 = G.| title = Cloning of thrombolytic enzyme (lumbrokinase) from earthworm and its expression in the yeast| journal = Protein Expression and Purification| volume = 42| issue = 1| pages = 20–8| year = 2005| pmid = 15927482| doi = 10.1016/j.pep.2005.04.005}}</ref>，且不同於[[鏈激酶]]等其他種抗血酸藥物，蚓激酶只有在[[纖維蛋白]]時才具有酵素活性，而較無造成{{link-en|纖溶亢進|hyperfibrinolysis}}導致{{link-en|大出血|hemorrhage}}之虞<Ref name=Wang2016/><ref>{{cite journal|title=Unearthing a source of medicinal molecules|author=Edwin L.Cooper, M.Balamurugan|journal=''Drug Discovery Today''|volume=15|issue=21-22|page=966-972|doi=10.1016/j.drudis.2010.09.004|year=2010}}</ref>。蚓激酶已在臨床上被用於治療[[中風]]與其他[[心血管疾病]]<Ref name=Wang2016/>。
+}}</ref><ref group="註">一種促進[[纖維蛋白]][[血栓|凝塊]]分解的蛋白質，能產生{{link-en|纖溶作用|fibrinolytic}}。</ref>，大小介於25-32[[原子质量单位|kDa]]之間<Ref name=Wang2016>{{Cite journal|title=Lumbrokinase attenuates myocardial ischemia-reperfusion injury by inhibiting TLR4 signaling|author=Wang YH, Chen KM, Chiu PS, Lai SC, Su HH, Jan MS, Lin CW, Lu DY, Fu YT, Liao JM, Chang JT, Huang SS|journal=''Journal of Molecular and Cellular Cardiology''|volume=99|page=113-122|doi=10.1016/j.yjmcc.2016.08.004|year=2016}}</ref>，可以多種途徑促進纖維蛋白的溶解，除了具有纖溶酶活性外，還可作為[[纖維蛋白溶酶原激活物]]以刺激纖溶酶的生成，又可促進組織間其他纖維蛋白溶酶原激活物的活性<Ref>{{cite journal|title=Cloning, Expression and Characterization of a Gene from Earthworm Eisenia fetida Encoding a Blood-Clot Dissolving Protein|author=GangQiang Li, Kevin Yueju Wang, DaHui Li, Nan Wang, DeHu Liu|journal=''PLoS One''|volume=7|issue=12|page=e53110|doi=10.1371/journal.pone.0053110}}</ref>。蚓激酶最早於1991年在{{link-en|正蚓屬|Lumbricus}}''[[Lumbricus bimastus]]''體內發現<Ref name=Wang2016/><Ref>{{cite journal|title=A Novel Fibrinolytic Enzyme Extracted from the Earthworm, Lumbricus rubellus|author=Hisashi MIHARA, Hiroyuki SUMI, Tomoyuki YONETA, Hideaki MIZUMOTO, Ryuzo IKEDA, Masao SEIKI, Masugi MARUYAMA|journal=''The Japanese Journal of Physiology''|volume=41|issue=3|doi=10.2170/jjphysiol.41.461|page=461-472}}</ref>，亦存在於{{link-en|赤子愛勝蚓|Eisenia fetida}}、{{link-en|粉正蚓|Lumbricus rubellus}}與等多種[[蚯蚓]]的[[體腔]]與[[消化道]]內，這種纖溶酶結構穩定，且在人體中可被[[小腸]]吸收，因而有作為{{link-en|抗血栓|antithrombotic}}藥物的潛力<ref>{{Cite journal| last1 = Ge | first1 = T.| last2 = Sun | first2 = Z.| last3 = Fu | first3 = S.| last4 = Liang | first4 = G.| title = Cloning of thrombolytic enzyme (lumbrokinase) from earthworm and its expression in the yeast| journal = Protein Expression and Purification| volume = 42| issue = 1| pages = 20–8| year = 2005| pmid = 15927482| doi = 10.1016/j.pep.2005.04.005}}</ref>，且不同於[[鏈激酶]]等其他種專一性較低的抗血酸藥物，蚓激酶對[[纖維蛋白]]的專一性很高，只有纖維蛋白存在時才具有酵素活性，而較無造成{{link-en|纖溶亢進|hyperfibrinolysis}}導致{{link-en|大出血|hemorrhage}}之虞<Ref name=Wang2016/><ref>{{cite journal|title=Unearthing a source of medicinal molecules|author=Edwin L.Cooper, M.Balamurugan|journal=''Drug Discovery Today''|volume=15|issue=21-22|page=966-972|doi=10.1016/j.drudis.2010.09.004|year=2010}}</ref>。蚓激酶已在臨床上被用於治療[[中風]]與其他[[心血管疾病]]<Ref name=Wang2016/>。
 ==註釋==