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Muse細胞：修订间差异

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'''Muse細胞'''（'''Mu'''lti-lineage differentiating '''s'''tress '''e'''nduring cell，多-{系}-分化持續應激細胞）是由[[日本東北大學]]出澤真理課題組於2010年發現一種內源性、無成瘤性的[[多能幹細胞]]<ref name="Kuroda">{{cite journal |doi=10.1073/pnas.0911647107 |pmid=20421459 |pmc=2889306 |title=Unique multipotent cells in adult human mesenchymal cell populations |journal=Proceedings of the National Academy of Sciences |volume=107 |issue=19 |pages=8639–43 |year=2010 |last1=Kuroda |first1=Y. |last2=Kitada |first2=M. |last3=Wakao |first3=S. |last4=Nishikawa |first4=K. |last5=Tanimura |first5=Y. |last6=Makinoshima |first6=H. |last7=Goda |first7=M. |last8=Akashi |first8=H. |last9=Inutsuka |first9=A. |last10=Niwa |first10=A. |last11=Shigemoto |first11=T. |last12=Nabeshima |first12=Y. |last13=Nakahata |first13=T. |last14=Nabeshima |first14=Y.-i. |last15=Fujiyoshi |first15=Y. |last16=Dezawa |first16=M. |bibcode=2010PNAS..107.8639K }}</ref>。幾乎所有不同器官來源的[[結締組織]]（如骨髓、外週血）中都有Muse細胞<ref name="Kuroda"/><ref name="WakaoPNAS">{{cite journal |doi=10.1073/pnas.1100816108 |pmid=21628574 |pmc=3116385 |title=Multilineage-differentiating stress-enduring (Muse) cells are a primary source of induced pluripotent stem cells in human fibroblasts |journal=Proceedings of the National Academy of Sciences |volume=108 |issue=24 |pages=9875–80 |year=2011 |last1=Wakao |first1=S. |last2=Kitada |first2=M. |last3=Kuroda |first3=Y. |last4=Shigemoto |first4=T. |last5=Matsuse |first5=D. |last6=Akashi |first6=H. |last7=Tanimura |first7=Y. |last8=Tsuchiyama |first8=K. |last9=Kikuchi |first9=T. |last10=Goda |first10=M. |last11=Nakahata |first11=T. |last12=Fujiyoshi |first12=Y. |last13=Dezawa |first13=M. |bibcode=2011PNAS..108.9875W }}</ref><ref name=":3">{{cite journal |doi=10.3727/096368916X690881 |pmid=26884346 |title=Muse Cells Provide the Pluripotency of Mesenchymal Stem Cells: Direct Contribution of Muse Cells to Tissue Regeneration |journal=Cell Transplantation |volume=25 |issue=5 |pages=849–61 |year=2016 |last1=Dezawa |first1=Mari }}</ref><ref name=":4">{{cite journal |doi=10.1016/j.jstrokecerebrovasdis.2015.12.033 |pmid=27019988 |title=Mobilization of Pluripotent Multilineage-Differentiating Stress-Enduring Cells in Ischemic Stroke |journal=Journal of Stroke and Cerebrovascular Diseases |volume=25 |issue=6 |pages=1473–81 |year=2016 |last1=Hori |first1=Emiko |last2=Hayakawa |first2=Yumiko |last3=Hayashi |first3=Tomohide |last4=Hori |first4=Satoshi |last5=Okamoto |first5=Soushi |last6=Shibata |first6=Takashi |last7=Kubo |first7=Michiya |last8=Horie |first8=Yukio |last9=Sasahara |first9=Masakiyo |last10=Kuroda |first10=Satoshi }}</ref>。通常Muse細胞可從商業化的間充質細胞中分離到，如人[[成纖維細胞]]、骨髓[[間充質幹細胞]]，以及脂肪源幹細胞（adipose-derived stem cells）<ref name=KurodaNature>{{cite journal |doi=10.1038/nprot.2013.076 |pmid=23787896 |title=Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cells |journal=Nature Protocols |volume=8 |issue=7 |pages=1391–415 |year=2013 |last1=Kuroda |first1=Yasumasa |last2=Wakao |first2=Shohei |last3=Kitada |first3=Masaaki |last4=Murakami |first4=Toru |last5=Nojima |first5=Makoto |last6=Dezawa |first6=Mari }}</ref><ref name="Heneidi">{{cite journal |doi=10.1371/journal.pone.0064752 |pmid=23755141 |pmc=3673968 |title=Awakened by Cellular Stress: Isolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue |journal=PLoS ONE |volume=8 |issue=6 |pages=e64752 |year=2013 |last1=Heneidi |first1=Saleh |last2=Simerman |first2=Ariel A. |last3=Keller |first3=Erica |last4=Singh |first4=Prapti |last5=Li |first5=Xinmin |last6=Dumesic |first6=Daniel A. |last7=Chazenbalk |first7=Gregorio |bibcode=2013PLoSO...864752H }}</ref>。Muse細胞可自發分化或在[[細胞因子]]誘導作用下分化爲三個胚層的細胞。Muse細胞的成瘤性較低，注入新的宿主體內也不會形成腫瘤，目前的觀點認爲這是因爲該細胞的[[端粒酶]]活性較低<ref name="Kuroda" />。
'''Muse細胞'''（'''Mu'''lti-lineage differentiating '''s'''tress '''e'''nduring cell，多-{系}-分化持續應激細胞）是由[[日本東北大學]]出澤真理課題組於2010年發現一種內源性、無成瘤性的[[多能幹細胞]]<ref name="Kuroda">{{cite journal |doi=10.1073/pnas.0911647107 |pmid=20421459 |pmc=2889306 |title=Unique multipotent cells in adult human mesenchymal cell populations |journal=Proceedings of the National Academy of Sciences |volume=107 |issue=19 |pages=8639–43 |year=2010 |last1=Kuroda |first1=Y. |last2=Kitada |first2=M. |last3=Wakao |first3=S. |last4=Nishikawa |first4=K. |last5=Tanimura |first5=Y. |last6=Makinoshima |first6=H. |last7=Goda |first7=M. |last8=Akashi |first8=H. |last9=Inutsuka |first9=A. |last10=Niwa |first10=A. |last11=Shigemoto |first11=T. |last12=Nabeshima |first12=Y. |last13=Nakahata |first13=T. |last14=Nabeshima |first14=Y.-i. |last15=Fujiyoshi |first15=Y. |last16=Dezawa |first16=M. |bibcode=2010PNAS..107.8639K }}</ref>。幾乎所有不同器官來源的[[結締組織]]（如骨髓、[[外周血]]）中都有Muse細胞<ref name="WakaoPNAS">{{cite journal |doi=10.1073/pnas.1100816108 |pmid=21628574 |pmc=3116385 |title=Multilineage-differentiating stress-enduring (Muse) cells are a primary source of induced pluripotent stem cells in human fibroblasts |journal=Proceedings of the National Academy of Sciences |volume=108 |issue=24 |pages=9875–80 |year=2011 |last1=Wakao |first1=S. |last2=Kitada |first2=M. |last3=Kuroda |first3=Y. |last4=Shigemoto |first4=T. |last5=Matsuse |first5=D. |last6=Akashi |first6=H. |last7=Tanimura |first7=Y. |last8=Tsuchiyama |first8=K. |last9=Kikuchi |first9=T. |last10=Goda |first10=M. |last11=Nakahata |first11=T. |last12=Fujiyoshi |first12=Y. |last13=Dezawa |first13=M. |bibcode=2011PNAS..108.9875W }}</ref><ref name=":3">{{cite journal |doi=10.3727/096368916X690881 |pmid=26884346 |title=Muse Cells Provide the Pluripotency of Mesenchymal Stem Cells: Direct Contribution of Muse Cells to Tissue Regeneration |journal=Cell Transplantation |volume=25 |issue=5 |pages=849–61 |year=2016 |last1=Dezawa |first1=Mari }}</ref><ref name=":4">{{cite journal |doi=10.1016/j.jstrokecerebrovasdis.2015.12.033 |pmid=27019988 |title=Mobilization of Pluripotent Multilineage-Differentiating Stress-Enduring Cells in Ischemic Stroke |journal=Journal of Stroke and Cerebrovascular Diseases |volume=25 |issue=6 |pages=1473–81 |year=2016 |last1=Hori |first1=Emiko |last2=Hayakawa |first2=Yumiko |last3=Hayashi |first3=Tomohide |last4=Hori |first4=Satoshi |last5=Okamoto |first5=Soushi |last6=Shibata |first6=Takashi |last7=Kubo |first7=Michiya |last8=Horie |first8=Yukio |last9=Sasahara |first9=Masakiyo |last10=Kuroda |first10=Satoshi }}</ref>。通常Muse細胞可從商業化的間充質細胞中分離到，如人[[成纖維細胞]]、骨髓[[間充質幹細胞]]，以及脂肪源幹細胞（adipose-derived stem cells）<ref name=KurodaNature>{{cite journal |doi=10.1038/nprot.2013.076 |pmid=23787896 |title=Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cells |journal=Nature Protocols |volume=8 |issue=7 |pages=1391–415 |year=2013 |last1=Kuroda |first1=Yasumasa |last2=Wakao |first2=Shohei |last3=Kitada |first3=Masaaki |last4=Murakami |first4=Toru |last5=Nojima |first5=Makoto |last6=Dezawa |first6=Mari }}</ref><ref name="Heneidi">{{cite journal |doi=10.1371/journal.pone.0064752 |pmid=23755141 |pmc=3673968 |title=Awakened by Cellular Stress: Isolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue |journal=PLoS ONE |volume=8 |issue=6 |pages=e64752 |year=2013 |last1=Heneidi |first1=Saleh |last2=Simerman |first2=Ariel A. |last3=Keller |first3=Erica |last4=Singh |first4=Prapti |last5=Li |first5=Xinmin |last6=Dumesic |first6=Daniel A. |last7=Chazenbalk |first7=Gregorio |bibcode=2013PLoSO...864752H }}</ref>。Muse細胞可自發分化或在[[細胞因子]]誘導作用下分化爲三個胚層的細胞。Muse細胞的成瘤性較低，注入新的宿主體內也不會形成腫瘤，目前的觀點認爲這是因爲該細胞的[[端粒酶]]活性較低<ref name="Kuroda" />。Muse細胞同時被應用於[[小鼠]]骨骼肌退變、[[糖尿病]]性皮膚潰瘍及急性重症肝炎等模型的修復研究中，研究發現Muse細胞在損傷組織中能夠和[[宿主]]的組織順利整合，並且分別向相應胚層的細胞分化<ref>{{cite journal |last1=Simerman |first1=AA |last2=Dumesic |first2=DA |last3=Chazenbalk |first3=GD |title=Pluripotent muse cells derived from human adipose tissue: a new perspective on regenerative medicine and cell therapy. |journal=Clinical and translational medicine |date=2014 |volume=3 |pages=12 |doi=10.1186/2001-1326-3-12 |pmid=24940477 |url=https://www.ncbi.nlm.nih.gov/m/pubmed/24940477/ |accessdate=2019-11-22}}</ref><ref>{{cite journal |last1=Wakao |first1=S |last2=Akashi |first2=H |last3=Kushida |first3=Y |last4=Dezawa |first4=M |title=Muse cells, newly found non-tumorigenic pluripotent stem cells, reside in human mesenchymal tissues. |journal=Pathology international |date=2014-01 |volume=64 |issue=1 |pages=1-9 |doi=10.1111/pin.12129 |pmid=24471964 |url=https://www.ncbi.nlm.nih.gov/m/pubmed/24471964: |accessdate=2019-11-22}}</ref><ref>{{cite journal |last1=Kuroda |first1=Y |last2=Dezawa |first2=M |title=Mesenchymal stem cells and their subpopulation, pluripotent muse cells, in basic research and regenerative medicine. |journal=Anatomical record (Hoboken, N.J. : 2007) |date=2014-01 |volume=297 |issue=1 |pages=98-110 |doi=10.1002/ar.22798 |pmid=24293378 |url=https://www.ncbi.nlm.nih.gov/m/pubmed/24293378/ |accessdate=2019-11-22}}</ref>。有研究利用Muse細胞修復腦動脈堵塞後，引起局部缺血的[[中風]]小鼠模型，發現Muse細胞修現可以替代丟失的[[神經元]]，整合至梗死的皮質組織中，分化為神經元標誌物表達陽性的細胞，並且有較為明顯的功能修復<ref>{{cite journal |last1=Yamauchi |first1=T |last2=Kuroda |first2=Y |last3=Morita |first3=T |last4=Shichinohe |first4=H |last5=Houkin |first5=K |last6=Dezawa |first6=M |last7=Kuroda |first7=S |title=Therapeutic effects of human multilineage-differentiating stress enduring (MUSE) cell transplantation into infarct brain of mice. |journal=PloS one |date=2015 |volume=10 |issue=3 |pages=e0116009 |doi=10.1371/journal.pone.0116009 |pmid=25747577 |url=https://www.ncbi.nlm.nih.gov/m/pubmed/25747577/ |accessdate=2019-11-22}}</ref>。


==培養==
==培養==

2019年11月22日 (五) 08:08的版本

Muse細胞（Multi-lineage differentiating stress enduring cell，多系分化持續應激細胞）是由日本東北大學出澤真理課題組於2010年發現一種內源性、無成瘤性的多能幹細胞[1]。幾乎所有不同器官來源的結締組織（如骨髓、外周血）中都有Muse細胞[2][3][4]。通常Muse細胞可從商業化的間充質細胞中分離到，如人成纖維細胞、骨髓間充質幹細胞，以及脂肪源幹細胞（adipose-derived stem cells）[5][6]。Muse細胞可自發分化或在細胞因子誘導作用下分化爲三個胚層的細胞。Muse細胞的成瘤性較低，注入新的宿主體內也不會形成腫瘤，目前的觀點認爲這是因爲該細胞的端粒酶活性較低[1]。Muse細胞同時被應用於小鼠骨骼肌退變、糖尿病性皮膚潰瘍及急性重症肝炎等模型的修復研究中，研究發現Muse細胞在損傷組織中能夠和宿主的組織順利整合，並且分別向相應胚層的細胞分化[7][8][9]。有研究利用Muse細胞修復腦動脈堵塞後，引起局部缺血的中風小鼠模型，發現Muse細胞修現可以替代丟失的神經元，整合至梗死的皮質組織中，分化為神經元標誌物表達陽性的細胞，並且有較為明顯的功能修復[10]。

培養

當Muse細胞被放置到含有營養因子和細胞因子的培養基中進行培養時，超過百分之九十的細胞都會進行定向細胞分化。若該培養基含有肝細胞生長因子、成纖維細胞生長因子4和地塞米松的胰島素-轉鐵蛋白-亞硒酸鈉時，百分之九十以上的細胞會分化成肝細胞，並且在α-胎兒蛋白和人類白蛋白表達中呈現陽性反應。在骨或脂肪細胞的培養基中，百分之九十八以上的細胞會分化為骨鈣蛋白或油紅O陽性的細胞[11]。

參見

參考資料

  1. ^ 1.0 1.1 Kuroda, Y.; Kitada, M.; Wakao, S.; Nishikawa, K.; Tanimura, Y.; Makinoshima, H.; Goda, M.; Akashi, H.; Inutsuka, A.; Niwa, A.; Shigemoto, T.; Nabeshima, Y.; Nakahata, T.; Nabeshima, Y.-i.; Fujiyoshi, Y.; Dezawa, M. Unique multipotent cells in adult human mesenchymal cell populations. Proceedings of the National Academy of Sciences. 2010, 107 (19): 8639–43. Bibcode:2010PNAS..107.8639K. PMC 2889306 可免费查阅. PMID 20421459. doi:10.1073/pnas.0911647107. 
  2. ^ Wakao, S.; Kitada, M.; Kuroda, Y.; Shigemoto, T.; Matsuse, D.; Akashi, H.; Tanimura, Y.; Tsuchiyama, K.; Kikuchi, T.; Goda, M.; Nakahata, T.; Fujiyoshi, Y.; Dezawa, M. Multilineage-differentiating stress-enduring (Muse) cells are a primary source of induced pluripotent stem cells in human fibroblasts. Proceedings of the National Academy of Sciences. 2011, 108 (24): 9875–80. Bibcode:2011PNAS..108.9875W. PMC 3116385 可免费查阅. PMID 21628574. doi:10.1073/pnas.1100816108. 
  3. ^ Dezawa, Mari. Muse Cells Provide the Pluripotency of Mesenchymal Stem Cells: Direct Contribution of Muse Cells to Tissue Regeneration. Cell Transplantation. 2016, 25 (5): 849–61. PMID 26884346. doi:10.3727/096368916X690881. 
  4. ^ Hori, Emiko; Hayakawa, Yumiko; Hayashi, Tomohide; Hori, Satoshi; Okamoto, Soushi; Shibata, Takashi; Kubo, Michiya; Horie, Yukio; Sasahara, Masakiyo; Kuroda, Satoshi. Mobilization of Pluripotent Multilineage-Differentiating Stress-Enduring Cells in Ischemic Stroke. Journal of Stroke and Cerebrovascular Diseases. 2016, 25 (6): 1473–81. PMID 27019988. doi:10.1016/j.jstrokecerebrovasdis.2015.12.033. 
  5. ^ Kuroda, Yasumasa; Wakao, Shohei; Kitada, Masaaki; Murakami, Toru; Nojima, Makoto; Dezawa, Mari. Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cells. Nature Protocols. 2013, 8 (7): 1391–415. PMID 23787896. doi:10.1038/nprot.2013.076. 
  6. ^ Heneidi, Saleh; Simerman, Ariel A.; Keller, Erica; Singh, Prapti; Li, Xinmin; Dumesic, Daniel A.; Chazenbalk, Gregorio. Awakened by Cellular Stress: Isolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue. PLoS ONE. 2013, 8 (6): e64752. Bibcode:2013PLoSO...864752H. PMC 3673968 可免费查阅. PMID 23755141. doi:10.1371/journal.pone.0064752. 
  7. ^ Simerman, AA; Dumesic, DA; Chazenbalk, GD. Pluripotent muse cells derived from human adipose tissue: a new perspective on regenerative medicine and cell therapy.. Clinical and translational medicine. 2014, 3: 12 [2019-11-22]. PMID 24940477. doi:10.1186/2001-1326-3-12. 
  8. ^ Wakao, S; Akashi, H; Kushida, Y; Dezawa, M. Muse cells, newly found non-tumorigenic pluripotent stem cells, reside in human mesenchymal tissues.. Pathology international. 2014-01, 64 (1): 1–9 [2019-11-22]. PMID 24471964. doi:10.1111/pin.12129. 
  9. ^ Kuroda, Y; Dezawa, M. Mesenchymal stem cells and their subpopulation, pluripotent muse cells, in basic research and regenerative medicine.. Anatomical record (Hoboken, N.J. : 2007). 2014-01, 297 (1): 98–110 [2019-11-22]. PMID 24293378. doi:10.1002/ar.22798. 
  10. ^ Yamauchi, T; Kuroda, Y; Morita, T; Shichinohe, H; Houkin, K; Dezawa, M; Kuroda, S. Therapeutic effects of human multilineage-differentiating stress enduring (MUSE) cell transplantation into infarct brain of mice.. PloS one. 2015, 10 (3): e0116009 [2019-11-22]. PMID 25747577. doi:10.1371/journal.pone.0116009. 
  11. ^ Ogura, Fumitaka; Wakao, Shohei; Kuroda, Yasumasa; Tsuchiyama, Kenichiro; Bagheri, Mozhdeh; Heneidi, Saleh; Chazenbalk, Gregorio; Aiba, Setsuya; Dezawa, Mari. Human Adipose Tissue Possesses a Unique Population of Pluripotent Stem Cells with Nontumorigenic and Low Telomerase Activities: Potential Implications in Regenerative Medicine. Stem Cells and Development. 2014, 23 (7): 717–28. PMID 24256547. doi:10.1089/scd.2013.0473. 
检索自“https://zh.wikipedia.org/w/index.php?title=Muse細胞&oldid=56971971”