DNA条形码

DNA条形码（英语：DNA barcoding）是一种利用基因组内的DNA片段来鉴定生物物种的技术。动物物种的鉴定采用线粒体细胞色素c氧化酶亚基I基因(cytochrome c oxidase I, COI)作为通用DNA条形码序列^[1]；生命条形码联盟(CBOL)植物工作组推荐叶绿体基因组的rbcL和matK片段作为植物鉴定的DNA条形码^[2]^[3]；生命条形码联盟真菌工作组推荐核糖体DNA内转录间隔区基因(nuclear ribosomal internal transcribed spacer, ITS)为真菌首选DNA条形码^[4]。

参考文献

^ Hebert, P. D. N.; Cywinska, A.; Ball, S. L.; deWaard, J. R. Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences. 2003-02-07, 270 (1512): 313–321. doi:10.1098/rspb.2002.2218.
^ Hollingsworth, P. M.; Forrest, L. L.; Spouge, J. L.; Hajibabaei, M.; Ratnasingham, S.; van der Bank, M.; Chase, M. W.; Cowan, R. S.; Erickson, D. L.; Fazekas, A. J.; Graham, S. W.; James, K. E.; Kim, K.-J.; Kress, W. J.; Schneider, H.; van AlphenStahl, J.; Barrett, S. C.H.; van den Berg, C.; Bogarin, D.; Burgess, K. S.; Cameron, K. M.; Carine, M.; Chacon, J.; Clark, A.; Clarkson, J. J.; Conrad, F.; Devey, D. S.; Ford, C. S.; Hedderson, T. A.J.; Hollingsworth, M. L.; Husband, B. C.; Kelly, L. J.; Kesanakurti, P. R.; Kim, J. S.; Kim, Y.-D.; Lahaye, R.; Lee, H.-L.; Long, D. G.; Madrinan, S.; Maurin, O.; Meusnier, I.; Newmaster, S. G.; Park, C.-W.; Percy, D. M.; Petersen, G.; Richardson, J. E.; Salazar, G. A.; Savolainen, V.; Seberg, O.; Wilkinson, M. J.; Yi, D.-K.; Little, D. P. A DNA barcode for land plants. Proceedings of the National Academy of Sciences. 2009-07-30, 106 (31): 12794–12797. doi:10.1073/pnas.0905845106.
^ Burgess, Kevin S.; Fazekas, Aron J.; Kesanakurti, Prasad R.; Graham, Sean W.; Husband, Brian C.; Newmaster, Steven G.; Percy, Diana M.; Hajibabaei, Mehrdad; Barrett, Spencer C. H. Discriminating plant species in a local temperate flora using the rbcL+matK DNA barcode. Methods in Ecology and Evolution. 2011-08, 2 (4): 333–340. doi:10.1111/j.2041-210X.2011.00092.x.
^ Pawlowski, Jan; Audic, Stéphane; Adl, Sina; Bass, David; Belbahri, Lassaâd; Berney, Cédric; Bowser, Samuel S.; Cepicka, Ivan; Decelle, Johan; Dunthorn, Micah; Fiore-Donno, Anna Maria; Gile, Gillian H.; Holzmann, Maria; Jahn, Regine; Jirků, Miloslav; Keeling, Patrick J.; Kostka, Martin; Kudryavtsev, Alexander; Lara, Enrique; Lukeš, Julius; Mann, David G.; Mitchell, Edward A. D.; Nitsche, Frank; Romeralo, Maria; Saunders, Gary W.; Simpson, Alastair G. B.; Smirnov, Alexey V.; Spouge, John L.; Stern, Rowena F.; Stoeck, Thorsten; Zimmermann, Jonas; Schindel, David; de Vargas, Colomban. CBOL Protist Working Group: Barcoding Eukaryotic Richness beyond the Animal, Plant, and Fungal Kingdoms. PLoS Biology. 2012-11-06, 10 (11): e1001419. doi:10.1371/journal.pbio.1001419.

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[1] Hebert, P. D. N.; Cywinska, A.; Ball, S. L.; deWaard, J. R. Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences. 2003-02-07, 270 (1512): 313–321. doi:10.1098/rspb.2002.2218.

[2] Hollingsworth, P. M.; Forrest, L. L.; Spouge, J. L.; Hajibabaei, M.; Ratnasingham, S.; van der Bank, M.; Chase, M. W.; Cowan, R. S.; Erickson, D. L.; Fazekas, A. J.; Graham, S. W.; James, K. E.; Kim, K.-J.; Kress, W. J.; Schneider, H.; van AlphenStahl, J.; Barrett, S. C.H.; van den Berg, C.; Bogarin, D.; Burgess, K. S.; Cameron, K. M.; Carine, M.; Chacon, J.; Clark, A.; Clarkson, J. J.; Conrad, F.; Devey, D. S.; Ford, C. S.; Hedderson, T. A.J.; Hollingsworth, M. L.; Husband, B. C.; Kelly, L. J.; Kesanakurti, P. R.; Kim, J. S.; Kim, Y.-D.; Lahaye, R.; Lee, H.-L.; Long, D. G.; Madrinan, S.; Maurin, O.; Meusnier, I.; Newmaster, S. G.; Park, C.-W.; Percy, D. M.; Petersen, G.; Richardson, J. E.; Salazar, G. A.; Savolainen, V.; Seberg, O.; Wilkinson, M. J.; Yi, D.-K.; Little, D. P. A DNA barcode for land plants. Proceedings of the National Academy of Sciences. 2009-07-30, 106 (31): 12794–12797. doi:10.1073/pnas.0905845106.

[3] Burgess, Kevin S.; Fazekas, Aron J.; Kesanakurti, Prasad R.; Graham, Sean W.; Husband, Brian C.; Newmaster, Steven G.; Percy, Diana M.; Hajibabaei, Mehrdad; Barrett, Spencer C. H. Discriminating plant species in a local temperate flora using the rbcL+matK DNA barcode. Methods in Ecology and Evolution. 2011-08, 2 (4): 333–340. doi:10.1111/j.2041-210X.2011.00092.x.

[4] Pawlowski, Jan; Audic, Stéphane; Adl, Sina; Bass, David; Belbahri, Lassaâd; Berney, Cédric; Bowser, Samuel S.; Cepicka, Ivan; Decelle, Johan; Dunthorn, Micah; Fiore-Donno, Anna Maria; Gile, Gillian H.; Holzmann, Maria; Jahn, Regine; Jirků, Miloslav; Keeling, Patrick J.; Kostka, Martin; Kudryavtsev, Alexander; Lara, Enrique; Lukeš, Julius; Mann, David G.; Mitchell, Edward A. D.; Nitsche, Frank; Romeralo, Maria; Saunders, Gary W.; Simpson, Alastair G. B.; Smirnov, Alexey V.; Spouge, John L.; Stern, Rowena F.; Stoeck, Thorsten; Zimmermann, Jonas; Schindel, David; de Vargas, Colomban. CBOL Protist Working Group: Barcoding Eukaryotic Richness beyond the Animal, Plant, and Fungal Kingdoms. PLoS Biology. 2012-11-06, 10 (11): e1001419. doi:10.1371/journal.pbio.1001419.

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