3-異丙基蘋果酸脫氫酶
外觀
3-異丙基蘋果酸脫氫酶 | |||||||||
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識別碼 | |||||||||
EC編號 | 1.1.1.85 | ||||||||
CAS號 | 9030-97-1 | ||||||||
數據庫 | |||||||||
IntEnz | IntEnz瀏覽 | ||||||||
BRENDA | BRENDA入口 | ||||||||
ExPASy | NiceZyme瀏覽 | ||||||||
KEGG | KEGG入口 | ||||||||
MetaCyc | 代謝路徑 | ||||||||
PRIAM | 概述 | ||||||||
PDB | RCSB PDB PDBj PDBe PDBsum | ||||||||
基因本體 | AmiGO / EGO | ||||||||
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3-異丙基蘋果酸脫氫酶(英語:3-isopropylmalate dehydrogenase,EC 1.1.1.85(頁面存檔備份,存於網際網路檔案館))是一種以NAD+或NADP+為受體、作用於供體CH-OH基團上的氧化還原酶。這種酶能催化以下酶促反應:
此反應實際上由兩步組成:
- (2S)-2-異丙基-3-氧代琥珀酸 ⇌ 4-甲基-2-氧代戊酸 + CO2
亦即上述第一步反應的產物可通過第二步反應自發脫羧產生4-甲基-2-氧代戊酸。
參考文獻
[編輯]- Burns RO, Umbarger HE, Gross, SR. The biosynthesis of leucine. III. The conversion of α-hydroxy-β-carboxyisocaproate to α-ketoisocaproate.. Biochemistry. 1963, 2: 1053–8. PMID 14087358. doi:10.1021/bi00905a024.
- Calvo JM, Stevens CM, Kalyanpur MG, Umbarger HE. the absolute configuration of alpha-hydroxy-beta-carboxyisocaproic acid (3-isopropylmalic acid), an intermediate in leucine biosynthesis. Biochemistry. December 1964, 3: 2024–7. PMID 14269331. doi:10.1021/bi00900a043.
- Parsons SJ, Burns RO. Purification and properties of beta-isopropylmalate dehydrogenase. J. Biol. Chem. February 1969, 244 (3): 996–1003. PMID 4889950.
- Németh A, Svingor A, Pócsik M, Dobó J, Magyar C, Szilágyi A, Gál P, Závodszky P. Mirror image mutations reveal the significance of an intersubunit ion cluster in the stability of 3-isopropylmalate dehydrogenase. FEBS Lett. February 2000, 468 (1): 48–52. PMID 10683439. doi:10.1016/S0014-5793(00)01190-X.