A-375
A-375 (亦称ATCC CRL-1619) 是人类恶性黑色素瘤细胞系，最初分离自一名54岁的女性恶性黑色素瘤患者[1]，并且有研究指出A-375细胞对Sutherlandia frutescens敏感[2]。该细胞系在经抗胸腺细胞球蛋白及血清处理的NIH瑞士小鼠中产生生长速度迅速的、类似黑色素瘤的皮下肿瘤。 同时应用于溶瘤肽LTX-315的溶瘤活性研究。它是具有62个染色体的二倍体细胞，每个细胞普遍存在着9个标记染色体，而且每个细胞以一个拷贝存在着正常的N2、N6和N22[1]。有研究指出抑制多种细胞增殖的新狼毒素A以时间及剂量依赖性的方式显著抑制A375细胞的生长和增殖，细胞体积变小且变得皱缩，核质浓缩且形成凋亡小体[3]。
参考资料[编辑]
- ^ 1.0 1.1 Giard, DJ; Aaronson, SA; Todaro, GJ; Arnstein, P; Kersey, JH; Dosik, H; Parks, WP. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.. Journal of the National Cancer Institute. 1973-11, 51 (5): 1417–23 [2019-12-06]. PMID 4357758. doi:10.1093/jnci/51.5.1417.
- ^ van der Walt, NB; Zakeri, Z; Cronjé, MJ. The Induction of Apoptosis in A375 Malignant Melanoma Cells by Sutherlandia frutescens.. Evidence-based complementary and alternative medicine : eCAM. 2016, 2016: 4921067 [2019-12-06]. PMID 27656236. doi:10.1155/2016/4921067.
- ^ Zhang, Chong; Zhou, Shuang-shuang; Feng, Lin-yi; Zhang, Da-yong; Lin, Neng-ming; Zhang, Li-huang; Pan, Jian-ping; Wang, Jun-bo; Li, Jie. In vitro anti-cancer activity of chamaejasmenin B and neochamaejasmin C isolated from the root of Stellera chamaejasme L. Acta Pharmacologica Sinica. 2012-12-10, 34 (2): 262–270 [2019-12-06]. doi:10.1038/aps.2012.158.