反式作用干扰小RNA
反式作用干扰小RNA（Trans-acting siRNA，简称tasiRNA）是陆生植物的一类小干扰RNA（siRNA），可进行RNA干扰，抑制其他基因的表现[1][2][3]。tasiRNA最早于2004年在模式生物阿拉伯芥中发现[1][2]，植物有TAS1、TAS2、TAS3与TAS4四个基因家族的基因编码tasiRNA，这些基因转录产生的RNA后会被多腺苷酸化，在miRNA介导之下TAS1、TAS2与TAS4转录的RNA5'端会被Ago1切割，TAS3转录的RNA则是3'端被Ago7切割[注 1][5]，接着此RNA由RNA复制酶RDR6转为双股RNA后，再被DCL4切割成长21nt且3'端有突出端的小双股RNA[6][7]，可与RNA诱导沉默复合体（RISC）结合以进行RNA干扰，将目标mRNA的切割移除[1]。
陆生植物有数种tasiRNA可抑制生长素应答因子（auxin response factor，ARF）的mRNA表现，其突变会造成植株发育异常[4]。
注脚[编辑]
参考文献[编辑]
- ^ 1.0 1.1 1.2 Vazquez F, Vaucheret H, Rajagopalan R, Lepers C, Gasciolli V, Mallory AC, Hilbert JL, Bartel DP, Crété P. Endogenous trans-acting siRNAs regulate the accumulation of Arabidopsis mRNAs. Mol. Cell. 2004, 16 (1): 69–79. PMID 15469823. doi:10.1016/j.molcel.2004.09.028.
- ^ 2.0 2.1 Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS. SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis. Genes Dev. 2004, 18 (19): 2368–79. PMC 522987
. PMID 15466488. doi:10.1101/gad.1231804.
- ^ Axtell MJ, Jan C, Rajagopalan R, Bartel DP. A two-hit trigger for siRNA biogenesis in plants. Cell. 2006, 127 (3): 565–77. PMID 17081978. doi:10.1016/j.cell.2006.09.032.
- ^ 4.0 4.1 de Felippes FF, Marchais A, Sarazin A, Oberlin S, Voinnet O. A single miR390 targeting event is sufficient for triggering TAS3-tasiRNA biogenesis in Arabidopsis.. Nucleic Acids Res. 2017, 45 (9): 5539–5554. PMC 5435969 . PMID 28334969. doi:10.1093/nar/gkx119.
- ^ Allen E, Howell MD. miRNAs in the biogenesis of trans-acting siRNAs in higher plants.. Semin Cell Dev Biol. 2010, 21 (8): 798–804. PMID 20359543. doi:10.1016/j.semcdb.2010.03.008.
- ^ Talmor-Neiman M, Stav R, Klipcan L, Buxdorf K, Baulcombe DC, Arazi T. Identification of trans-acting siRNAs in moss and an RNA-dependent RNA polymerase required for their biogenesis. Plant J. 2006, 48 (4): 511–21. PMID 17076803. doi:10.1111/j.1365-313X.2006.02895.x.
- ^ Rajeswaran R, Aregger M, Zvereva AS, Borah BK, Gubaeva EG, Pooggin MM. Sequencing of RDR6-dependent double-stranded RNAs reveals novel features of plant siRNA biogenesis.. Nucleic Acids Res. 2012, 40 (13): 6241–54. PMC 3401431 . PMID 22434877. doi:10.1093/nar/gks242.
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