CD2AP

CD2相关蛋白质
(CD2-associated protein)
CD2相关蛋白质; (CD2-associated protein)
	基于2j6f的PDB绘制。
有效结构
PDB	直系同源检索：PDBe, RCSB
PDB查询代码列表
	2FEI, 2J6F, 2J6K, 2J6O, 2J7I, 3AA6, 3LK4, 3U23
标识
代号	CD2AP; CMS
扩展标识	遗传学：604241 鼠基因：1330281 同源基因：7663 GeneCards: CD2AP Gene
基因本体论描述
分子功能	· vascular endothelial growth factor receptor binding; · structural constituent of cytoskeleton; · protein binding; · beta-catenin binding; · protein C-terminus binding; · SH3 domain binding; · protein complex binding; · cadherin binding;
细胞成分	· ruffle; · cytoplasm; · plasma membrane; · actin cytoskeleton; · endocytic vesicle; · filamentous actin; · perinuclear region of cytoplasm; · extracellular vesicular exosome;
生物过程	· protein complex assembly; · substrate-dependent cell migration, cell extension; · mitotic nuclear division; · signal transduction; · vesicle organization; · single organismal cell-cell adhesion; · proteasome-mediated ubiquitin-dependent protein catabolic process; · regulation of receptor-mediated endocytosis;
	Sources: Amigo / QuickGO
直系同源体
物种	人类
Entrez	23607
Ensembl	ENSG00000198087
UniProt	Q9Y5K6
mRNA序列	NM_012120
蛋白序列	NP_036252
基因位置	Chr 6:; 47.45 – 47.59 Mb
PubMed查询	[1]
	查; 论; 编;

CD2相关蛋白质(CD2-associated protein)是在人类中由CD2AP基因所编码的蛋白质。^[1]^[2]

该基因编码一种调节肌动蛋白细胞骨架的"支架分子"(在神经元通路中的广大连结区域聚积的蛋白分子)。该蛋白质直接与丝状肌动蛋白、及通过多种肌动蛋白结合位点的各种细胞膜的蛋白质、SH3结构域(SH3 domain)，以及含有SH3结构域结合位点之"富含脯氨酸区域"(proline-rich region)等交互作用。胞质蛋白定位于膜皱褶(Membrane ruffling)，脂筏(Lipid raft)和细胞的前缘。它牵涉到在受体的内吞作用和"胞质分裂"(cytokinesis)发生动态肌动蛋白重塑(actin remodeling)和膜交换。该基因的"单倍剂量不足"(Haploinsufficiency)牵涉到易感性的肾小球疾病。^[2]

交互作用

CD2AP已显示出与NPHS2、^[3]肾病蛋白、^[4] CBL基因(CBL (gene))，^[5]^[6]^[7] 及RAB4A^[7]等有蛋白质交互作用

参见

局灶节段性肾小球硬化症

注释

^ Kirsch KH, Georgescu MM, Ishimaru S, Hanafusa H. CMS: An adapter molecule involved in cytoskeletal rearrangements. Proc Natl Acad Sci USA. Jun 1999, 96 (11): 6211–6. PMC 26861 . PMID 10339567. doi:10.1073/pnas.96.11.6211.
^ ^2.0 ^2.1 Entrez Gene: CD2AP CD2-associated protein. （原始内容存档于2010-03-08）.
^ Schwarz, K; Simons M; Reiser J; Saleem M A; Faul C; Kriz W; Shaw A S; Holzman L B; Mundel P. Podocin, a raft-associated component of the glomerular slit diaphragm, interacts with CD2AP and nephrin. J. Clin. Invest. (United States). Dec 2001, 108 (11): 1621–9. ISSN 0021-9738. PMC 200981 . PMID 11733557. doi:10.1172/JCI12849.
^ Lehtonen, Sanna; Lehtonen Eero; Kudlicka Krystyna; Holthöfer Harry; Farquhar Marilyn G. Nephrin Forms a Complex with Adherens Junction Proteins and CASK in Podocytes and in Madin-Darby Canine Kidney Cells Expressing Nephrin. Am. J. Pathol. (United States). Sep 2004, 165 (3): 923–36. ISSN 0002-9440. PMC 1618613 . PMID 15331416. doi:10.1016/S0002-9440(10)63354-8.
^ Haglund, Kaisa; Ivankovic-Dikic Inga; Shimokawa Noriaki; Kruh Gary D; Dikic Ivan. Recruitment of Pyk2 and Cbl to lipid rafts mediates signals important for actin reorganization in growing neurites. J. Cell. Sci. (England). May 2004, 117 (Pt 12): 2557–68. ISSN 0021-9533. PMID 15128873. doi:10.1242/jcs.01148.
^ Kirsch, K H; Georgescu M M; Shishido T; Langdon W Y; Birge R B; Hanafusa H. The adapter type protein CMS/CD2AP binds to the proto-oncogenic protein c-Cbl through a tyrosine phosphorylation-regulated Src homology 3 domain interaction. J. Biol. Chem. (United States). Feb 2001, 276 (7): 4957–63. ISSN 0021-9258. PMID 11067845. doi:10.1074/jbc.M005784200.
^ ^7.0 ^7.1 Cormont, Mireille; Metón Isidoro; Mari Muriel; Monzo Pascale; Keslair Frédérique; Gaskin Chantell; McGraw Timothy E; Le Marchand-Brustel Yannick. CD2AP/CMS regulates endosome morphology and traffic to the degradative pathway through its interaction with Rab4 and c-Cbl. Traffic (Denmark). Feb 2003, 4 (2): 97–112. ISSN 1398-9219. PMID 12559036. doi:10.1034/j.1600-0854.2003.40205.x.

延伸阅读

Bonaldo MF, Lennon G, Soares MB. Normalization and subtraction: two approaches to facilitate gene discovery. Genome Res. 1997, 6 (9): 791–806. PMID 8889548. doi:10.1101/gr.6.9.791.
Dustin ML; Olszowy MW; Holdorf AD; et al. A novel adaptor protein orchestrates receptor patterning and cytoskeletal polarity in T-cell contacts. Cell. 1998, 94 (5): 667–77. PMID 9741631. doi:10.1016/S0092-8674(00)81608-6.
Lehtonen S; Ora A; Olkkonen VM; et al. In vivo interaction of the adapter protein CD2-associated protein with the type 2 polycystic kidney disease protein, polycystin-2. J. Biol. Chem. 2000, 275 (42): 32888–93. PMID 10913159. doi:10.1074/jbc.M006624200.
Li C; Ruotsalainen V; Tryggvason K; et al. CD2AP is expressed with nephrin in developing podocytes and is found widely in mature kidney and elsewhere. Am. J. Physiol. Renal Physiol. 2000, 279 (4): F785–92. PMID 10997929.
Kirsch KH; Georgescu MM; Shishido T; et al. The adapter type protein CMS/CD2AP binds to the proto-oncogenic protein c-Cbl through a tyrosine phosphorylation-regulated Src homology 3 domain interaction. J. Biol. Chem. 2001, 276 (7): 4957–63. PMID 11067845. doi:10.1074/jbc.M005784200.
Schwarz K; Simons M; Reiser J; et al. Podocin, a raft-associated component of the glomerular slit diaphragm, interacts with CD2AP and nephrin. J. Clin. Invest. 2002, 108 (11): 1621–9. PMC 200981 . PMID 11733557. doi:10.1172/JCI12849.
Scott MP; Zappacosta F; Kim EY; et al. Identification of novel SH3 domain ligands for the Src family kinase Hck. Wiskott-Aldrich syndrome protein (WASP), WASP-interacting protein (WIP), and ELMO1. J. Biol. Chem. 2002, 277 (31): 28238–46. PMID 12029088. doi:10.1074/jbc.M202783200.
Strausberg RL; Feingold EA; Grouse LH; et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. U.S.A. 2003, 99 (26): 16899–903. PMC 139241 . PMID 12477932. doi:10.1073/pnas.242603899.
Badour K; Zhang J; Shi F; et al. The Wiskott-Aldrich syndrome protein acts downstream of CD2 and the CD2AP and PSTPIP1 adaptors to promote formation of the immunological synapse. Immunity. 2003, 18 (1): 141–54. PMID 12530983. doi:10.1016/S1074-7613(02)00516-2.
Cormont M; Metón I; Mari M; et al. CD2AP/CMS regulates endosome morphology and traffic to the degradative pathway through its interaction with Rab4 and c-Cbl. Traffic. 2003, 4 (2): 97–112. PMID 12559036. doi:10.1034/j.1600-0854.2003.40205.x.
Lynch DK; Winata SC; Lyons RJ; et al. A Cortactin-CD2-associated protein (CD2AP) complex provides a novel link between epidermal growth factor receptor endocytosis and the actin cytoskeleton. J. Biol. Chem. 2003, 278 (24): 21805–13. PMID 12672817. doi:10.1074/jbc.M211407200.
Hutchings NJ; Clarkson N; Chalkley R; et al. Linking the T cell surface protein CD2 to the actin-capping protein CAPZ via CMS and CIN85. J. Biol. Chem. 2003, 278 (25): 22396–403. PMID 12690097. doi:10.1074/jbc.M302540200.
Kim JM; Wu H; Green G; et al. CD2-associated protein haploinsufficiency is linked to glomerular disease susceptibility. Science. 2003, 300 (5623): 1298–300. PMID 12764198. doi:10.1126/science.1081068.
Schulze WX, Mann M. A novel proteomic screen for peptide-protein interactions. J. Biol. Chem. 2004, 279 (11): 10756–64. PMID 14679214. doi:10.1074/jbc.M309909200.
Haglund K; Ivankovic-Dikic I; Shimokawa N; et al. Recruitment of Pyk2 and Cbl to lipid rafts mediates signals important for actin reorganization in growing neurites. J. Cell. Sci. 2004, 117 (Pt 12): 2557–68. PMID 15128873. doi:10.1242/jcs.01148.
Lehtonen S; Lehtonen E; Kudlicka K; et al. Nephrin Forms a Complex with Adherens Junction Proteins and CASK in Podocytes and in Madin-Darby Canine Kidney Cells Expressing Nephrin. Am. J. Pathol. 2004, 165 (3): 923–36. PMC 1618613 . PMID 15331416. doi:10.1016/S0002-9440(10)63354-8.
Gerhard DS; Wagner L; Feingold EA; et al. The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC). Genome Res. 2004, 14 (10B): 2121–7. PMC 528928 . PMID 15489334. doi:10.1101/gr.2596504.
Rush J; Moritz A; Lee KA; et al. Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. Nat. Biotechnol. 2005, 23 (1): 94–101. PMID 15592455. doi:10.1038/nbt1046.
Coward RJ; Foster RR; Patton D; et al. Nephrotic plasma alters slit diaphragm-dependent signaling and translocates nephrin, Podocin, and CD2 associated protein in cultured human podocytes. J. Am. Soc. Nephrol. 2005, 16 (3): 629–37. PMID 15659563. doi:10.1681/ASN.2004030172.

[pmid10339567-1] Kirsch KH, Georgescu MM, Ishimaru S, Hanafusa H. CMS: An adapter molecule involved in cytoskeletal rearrangements. Proc Natl Acad Sci USA. Jun 1999, 96 (11): 6211–6. PMC 26861 . PMID 10339567. doi:10.1073/pnas.96.11.6211.

[entrez-2] 2.0 ^2.1 Entrez Gene: CD2AP CD2-associated protein. （原始内容存档于2010-03-08）.

[pmid11733557-3] Schwarz, K; Simons M; Reiser J; Saleem M A; Faul C; Kriz W; Shaw A S; Holzman L B; Mundel P. Podocin, a raft-associated component of the glomerular slit diaphragm, interacts with CD2AP and nephrin. J. Clin. Invest. (United States). Dec 2001, 108 (11): 1621–9. ISSN 0021-9738. PMC 200981 . PMID 11733557. doi:10.1172/JCI12849.

[pmid15331416-4] Lehtonen, Sanna; Lehtonen Eero; Kudlicka Krystyna; Holthöfer Harry; Farquhar Marilyn G. Nephrin Forms a Complex with Adherens Junction Proteins and CASK in Podocytes and in Madin-Darby Canine Kidney Cells Expressing Nephrin. Am. J. Pathol. (United States). Sep 2004, 165 (3): 923–36. ISSN 0002-9440. PMC 1618613 . PMID 15331416. doi:10.1016/S0002-9440(10)63354-8.

[pmid15128873-5] Haglund, Kaisa; Ivankovic-Dikic Inga; Shimokawa Noriaki; Kruh Gary D; Dikic Ivan. Recruitment of Pyk2 and Cbl to lipid rafts mediates signals important for actin reorganization in growing neurites. J. Cell. Sci. (England). May 2004, 117 (Pt 12): 2557–68. ISSN 0021-9533. PMID 15128873. doi:10.1242/jcs.01148.

[pmid11067845-6] Kirsch, K H; Georgescu M M; Shishido T; Langdon W Y; Birge R B; Hanafusa H. The adapter type protein CMS/CD2AP binds to the proto-oncogenic protein c-Cbl through a tyrosine phosphorylation-regulated Src homology 3 domain interaction. J. Biol. Chem. (United States). Feb 2001, 276 (7): 4957–63. ISSN 0021-9258. PMID 11067845. doi:10.1074/jbc.M005784200.

[pmid12559036-7] 7.0 ^7.1 Cormont, Mireille; Metón Isidoro; Mari Muriel; Monzo Pascale; Keslair Frédérique; Gaskin Chantell; McGraw Timothy E; Le Marchand-Brustel Yannick. CD2AP/CMS regulates endosome morphology and traffic to the degradative pathway through its interaction with Rab4 and c-Cbl. Traffic (Denmark). Feb 2003, 4 (2): 97–112. ISSN 1398-9219. PMID 12559036. doi:10.1034/j.1600-0854.2003.40205.x.

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