主要碱性蛋白

主要碱性蛋白
已知的结构
PDB	直系同源搜索: PDBe RCSB
PDBID列表
	1H8U、2BRS、4QXX
识别号
别名	PRG2；, BMPG, MBP, MBP1, proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein), proMBP, proteoglycan 2, pro eosinophil major basic protein
外部ID	OMIM：605601 MGI：103294 HomoloGene：2044 GeneCards：PRG2
基因位置（人类）
染色体	11号染色体
终止	57,390,650 bp
基因位置（小鼠）
染色体	小鼠2号染色体
终止	84,813,976 bp
RNA表达模式
	查阅更多表达数据
基因本体
分子功能	• heparin binding; • carbohydrate binding; • extracellular matrix structural constituent conferring compression resistance;
细胞组分	• 细胞外区域; • transport vesicle; • cytoplasmic vesicle; • 外排体; • ficolin-1-rich granule lumen; • collagen-containing extracellular matrix;
生物学过程	• defense response to bacterium; • immune system process; • defense response to nematode; • negative regulation of interleukin-10 production; • positive regulation of interleukin-4 production; • 免疫反应; • neutrophil degranulation;
	Sources:Amigo / QuickGO
直系同源
	5553
	19074
	ENSG00000186652
	ENSMUSG00000027073
	P13727
	Q61878
	NM_002728; NM_001243245; NM_001302926; NM_001302927
	NM_008920
	NP_001230174; NP_001289855; NP_001289856; NP_002719
	NP_032946
	维基数据
查看/编辑人类	查看/编辑小鼠

主要碱性蛋白（英语：Major basic protein，MBP），全称为嗜酸性粒细胞主要碱性蛋白（英语：Eosinophil Major basic protein），也称为蛋白多糖2（Proteoglycan 2，PRG2），是一种由人类基因PRG2编码的蛋白质^[5]。

MBP在结构上类似于凝集素（糖结合蛋白），特别是与C-型凝集素在折叠上相似。但与C-型凝集素不同（C-型凝集素在钙的存在下会结合各种碳水化合物），MBP既不与钙结合也不与这些糖类结合，而是识别并结合硫酸乙酰肝素（heparan sulfate）。MBP的两种晶体结构已被测定。^[6]^[7]

相互作用

主要碱性蛋白已知可与妊娠相关血浆蛋白A发生蛋白质交互作用。^[8]^[9]^[10]

参考文献

^ ^1.0 ^1.1 ^1.2 GRCh38: Ensembl release 89: ENSG00000186652 - Ensembl, May 2017
^ ^2.0 ^2.1 ^2.2 GRCm38: Ensembl release 89: ENSMUSG00000027073 - Ensembl, May 2017
^ Human PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Mouse PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Entrez Gene: PRG2 proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein). （原始内容存档于2008-11-02）.
^ PDB 1h8u; Swaminathan GJ, Weaver AJ, Loegering DA, Checkel JL, Leonidas DD, Gleich GJ, Acharya KR. Crystal structure of the eosinophil major basic protein at 1.8 A. An atypical lectin with a paradigm shift in specificity. J. Biol. Chem. July 2001, 276 (28): 26197–26203. PMID 11319227. doi:10.1074/jbc.M100848200.
^ PDB 2brs; Swaminathan GJ, Myszka DG, Katsamba PS, Ohnuki LE, Gleich GJ, Acharya KR. Eosinophil-granule major basic protein, a C-type lectin, binds heparin. Biochemistry. November 2005, 44 (43): 14152–14158. PMID 16245931. doi:10.1021/bi051112b.
^ Overgaard, M T; Haaning J; Boldt H B; Olsen I M; Laursen L S; Christiansen M; Gleich G J; Sottrup-Jensen L; Conover C A; Oxvig C. Expression of recombinant human pregnancy-associated plasma protein-A and identification of the proform of eosinophil major basic protein as its physiological inhibitor. J. Biol. Chem. (UNITED STATES). October 2000, 275 (40): 31128–31133. ISSN 0021-9258. PMID 10913121. doi:10.1074/jbc.M001384200.
^ Overgaard MT, Sorensen ES, Stachowiak D, Boldt HB, Kristensen L, Sottrup-Jensen L, Oxvig C. Complex of pregnancy-associated plasma protein-A and the proform of eosinophil major basic protein. Disulfide structure and carbohydrate attachment. J. Biol. Chem. (United States). January 2003, 278 (4): 2106–2117. ISSN 0021-9258. PMID 12421832. doi:10.1074/jbc.M208777200.
^ Oxvig, C; Sand O; Kristensen T; Gleich G J; Sottrup-Jensen L. Circulating human pregnancy-associated plasma protein-A is disulfide-bridged to the proform of eosinophil major basic protein. J. Biol. Chem. (UNITED STATES). June 1993, 268 (17): 12243–6. ISSN 0021-9258. PMID 7685339.

外部链接

医学主题词表（MeSH）：Eosinophil+Major+Basic+Protein

延伸阅读

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Barker RL, Loegering DA, Arakawa KC, et al. Cloning and sequence analysis of the human gene encoding eosinophil major basic protein. Gene. 1990, 86 (2): 285–289. PMID 2323577. doi:10.1016/0378-1119(90)90292-Y.
Gabay JE, Scott RW, Campanelli D, et al. Antibiotic proteins of human polymorphonuclear leukocytes. Proc. Natl. Acad. Sci. U.S.A. 1989, 86 (14): 5610–5614. Bibcode:1989PNAS...86.5610G. PMC 297672 . PMID 2501794. doi:10.1073/pnas.86.14.5610.
Wasmoen TL, McKean DJ, Benirschke K, et al. Evidence of eosinophil granule major basic protein in human placenta. J. Exp. Med. 1990, 170 (6): 2051–2063. PMC 2189540 . PMID 2584934. doi:10.1084/jem.170.6.2051.
Barker RL, Gleich GJ, Pease LR. Acidic precursor revealed in human eosinophil granule major basic protein cDNA. J. Exp. Med. 1988, 168 (4): 1493–1498. PMC 2189086 . PMID 3171483. doi:10.1084/jem.168.4.1493.
McGrogan M, Simonsen C, Scott R, et al. Isolation of a complementary DNA clone encoding a precursor to human eosinophil major basic protein. J. Exp. Med. 1989, 168 (6): 2295–2308. PMC 2189145 . PMID 3199069. doi:10.1084/jem.168.6.2295.
Wasmoen TL, Bell MP, Loegering DA, et al. Biochemical and amino acid sequence analysis of human eosinophil granule major basic protein. J. Biol. Chem. 1988, 263 (25): 12559–63. PMID 3410852.
Weller PF, Ackerman SJ, Smith JA. Eosinophil granule cationic proteins: major basic protein is distinct from the smaller subunit of eosinophil peroxidase. J. Leukoc. Biol. 1988, 43 (1): 1–4. PMID 3422083.
Kristensen T, Oxvig C, Sand O, et al. Amino acid sequence of human pregnancy-associated plasma protein-A derived from cloned cDNA. Biochemistry. 1994, 33 (6): 1592–1598. PMID 7508748. doi:10.1021/bi00172a040.
Oxvig C, Haaning J, Højrup P, Sottrup-Jensen L. Location and nature of carbohydrate groups in proform of human major basic protein isolated from pregnancy serum. Biochem. Mol. Biol. Int. 1994, 33 (2): 329–36. PMID 7524900.
Bonno M, Oxvig C, Kephart GM, et al. Localization of pregnancy-associated plasma protein-A and colocalization of pregnancy-associated plasma protein-A messenger ribonucleic acid and eosinophil granule major basic protein messenger ribonucleic acid in placenta. Lab. Invest. 1994, 71 (4): 560–6. PMID 7526035.
Li MS, Sun L, Satoh T, et al. Human eosinophil major basic protein, a mediator of allergic inflammation, is expressed by alternative splicing from two promoters. Biochem. J. 1995, 305 (3): 921–7. PMC 1136346 . PMID 7531438.
Oxvig C, Haaning J, Kristensen L, et al. Identification of angiotensinogen and complement C3dg as novel proteins binding the proform of eosinophil major basic protein in human pregnancy serum and plasma. J. Biol. Chem. 1995, 270 (23): 13645–13651. PMID 7539791. doi:10.1074/jbc.270.23.13645.
Oxvig C, Sand O, Kristensen T, et al. Circulating human pregnancy-associated plasma protein-A is disulfide-bridged to the proform of eosinophil major basic protein. J. Biol. Chem. 1993, 268 (17): 12243–6. PMID 7685339.
Levi-Schaffer F, Lacy P, Severs NJ, et al. Association of granulocyte-macrophage colony-stimulating factor with the crystalloid granules of human eosinophils. Blood. 1995, 85 (9): 2579–86. PMID 7727786.
Oxvig C, Gleich GJ, Sottrup-Jensen L. Localization of disulfide bridges and free sulfhydryl groups in human eosinophil granule major basic protein. FEBS Lett. 1994, 341 (2–3): 213–217. PMID 8137941. doi:10.1016/0014-5793(94)80459-1.
Shikata Y, Hayashi Y, Yoshimatsu K, et al. Pro-major basic protein has three types of sugar chains at the pro-portion. Biochim. Biophys. Acta. 1993, 1163 (3): 243–9. PMID 8507662. doi:10.1016/0167-4838(93)90158-N.
Nittoh T, Watanabe M, Okayama H, et al. Cloning of cDNA for rat eosinophil major basic protein. Biochim. Biophys. Acta. 1996, 1264 (3): 261–4. PMID 8547309. doi:10.1016/0167-4781(95)00183-2.
Delcommenne M, Tan C, Gray V, et al. Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase. Proc. Natl. Acad. Sci. U.S.A. 1998, 95 (19): 11211–11216. Bibcode:1998PNAS...9511211D. PMC 21621 . PMID 9736715. doi:10.1073/pnas.95.19.11211.

[refGRCh38Ensembl-1] 1.0 ^1.1 ^1.2 GRCh38: Ensembl release 89: ENSG00000186652 - Ensembl, May 2017

[refGRCm38Ensembl-2] 2.0 ^2.1 ^2.2 GRCm38: Ensembl release 89: ENSMUSG00000027073 - Ensembl, May 2017

[3] Human PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] Mouse PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.

[entrez-5] Entrez Gene: PRG2 proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein). （原始内容存档于2008-11-02）.

[pmid11319227-6] PDB 1h8u; Swaminathan GJ, Weaver AJ, Loegering DA, Checkel JL, Leonidas DD, Gleich GJ, Acharya KR. Crystal structure of the eosinophil major basic protein at 1.8 A. An atypical lectin with a paradigm shift in specificity. J. Biol. Chem. July 2001, 276 (28): 26197–26203. PMID 11319227. doi:10.1074/jbc.M100848200.

[pmid16245931-7] PDB 2brs; Swaminathan GJ, Myszka DG, Katsamba PS, Ohnuki LE, Gleich GJ, Acharya KR. Eosinophil-granule major basic protein, a C-type lectin, binds heparin. Biochemistry. November 2005, 44 (43): 14152–14158. PMID 16245931. doi:10.1021/bi051112b.

[pmid10913121-8] Overgaard, M T; Haaning J; Boldt H B; Olsen I M; Laursen L S; Christiansen M; Gleich G J; Sottrup-Jensen L; Conover C A; Oxvig C. Expression of recombinant human pregnancy-associated plasma protein-A and identification of the proform of eosinophil major basic protein as its physiological inhibitor. J. Biol. Chem. (UNITED STATES). October 2000, 275 (40): 31128–31133. ISSN 0021-9258. PMID 10913121. doi:10.1074/jbc.M001384200.

[pmid12421832-9] Overgaard MT, Sorensen ES, Stachowiak D, Boldt HB, Kristensen L, Sottrup-Jensen L, Oxvig C. Complex of pregnancy-associated plasma protein-A and the proform of eosinophil major basic protein. Disulfide structure and carbohydrate attachment. J. Biol. Chem. (United States). January 2003, 278 (4): 2106–2117. ISSN 0021-9258. PMID 12421832. doi:10.1074/jbc.M208777200.

[pmid7685339-10] Oxvig, C; Sand O; Kristensen T; Gleich G J; Sottrup-Jensen L. Circulating human pregnancy-associated plasma protein-A is disulfide-bridged to the proform of eosinophil major basic protein. J. Biol. Chem. (UNITED STATES). June 1993, 268 (17): 12243–6. ISSN 0021-9258. PMID 7685339.

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