异柠檬酸裂合酶

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Isocitrate Lyase
Isocitrate lyase.png
Homotetrameric structure of Isocitrate lyase from E. coli. Based on PDB 1IGW.[1]
识别码
EC编号 4.1.3.1
CAS号 9045-78-7
数据库
IntEnz IntEnz浏览
BRENDA英语BRENDA BRENDA入口
ExPASy英语ExPASy NiceZyme浏览
KEGG KEGG入口
MetaCyc英语MetaCyc 代谢路径
PRIAM英语PRIAM_enzyme-specific_profiles 概述
PDB RCSB PDB PDBj PDBe PDBsum
基因本体 AmiGO / EGO
Isocitrate lyase family
鑑定
標誌 ICL
Pfam PF00463
InterPro英语InterPro IPR000918
PROSITE英语PROSITE PDOC00145
 SCOP英语Structural Classification of Proteins 1f8m / SUPFAM

异柠檬酸裂合酶(英語:Isocitrate lyase,缩写ICLEC 4.1.3.1)是以一种在乙醛酸循环中将异柠檬酸切割为乙醛酸琥珀酸[2][3]。其产物再通过苹果酸合酶合成苹果酸,跳过了三羧酸循环(TCA循环)中脱去CO2的两步。这一途径广泛存在于细菌、真菌与植物中。[4]

机制[编辑]

异柠檬酸裂合酶属于异柠檬酸裂合酶家族英语Isocitrate lyase family,该家族的其它成员有羧乙烯基-羧基膦酸酯磷酰基变位酶英语carboxyvinyl-carboxyphosphonate phosphorylmutaseEC 2.7.8.23),以及磷酸烯醇式丙酮酸变位酶EC 5.4.2.9,参与草丁膦类三肽抗生素的合成)。

ICL-catalyzed reaction

在乙醛酸循环中,苹果酸合酶可催化乙酰辅酶A与乙醛酸缩合形成苹果酸从而使循环得以继续。

酶结构[编辑]

截止2007年底,已有5个这类酶的结构被解析,PDB接入代码为1DQU1F611F8I1F8M1IGW

另见[编辑]

参考文献[编辑]

  1. ^ Britton, KL; Abeysinghe IS, Baker PJ, Barynin V, Diehl P, Langridge SJ, McFadden BA, Sedelnikova SE, Stillman TJ, Weeradechapon K, Rice DW. The structure and domain organization of Escherichia coli isocitrate lyase. Acta Crystallogr D. Sep 2001, 57 (9): 1209–1218. PMID 11526312. doi:10.1107/S0907444901008642. 
  2. ^ Beeching JR. High sequence conservation between isocitrate lyase from Escherichia coli and Ricinus communis. Protein Seq. Data Anal. 1989, 2 (6): 463–466. PMID 2696959. 
  3. ^ Tanaka A, Atomi H, Ueda M, Hikida M, Hishida T, Teranishi Y. Peroxisomal isocitrate lyase of the n-alkane-assimilating yeast Candida tropicalis: gene analysis and characterization. J. Biochem. 1990, 107 (2): 262–266. PMID 2361956. 
  4. ^ Dunn, MF; Ramirez-Trujill JA; Hernandez-Lucas I. Major roles of isocitrate lyase and malate synthase in bacterial and fungal pathogenesis. Microbiology. Oct 2009, 155 (10): 3166–3175. PMID 19684068. doi:10.1099/mic.0.030858-0. 

延伸阅读[编辑]

  • McFadden BA and Howes WV. Crystallisation and some properties of isocitrate lyase from Pseudomonas indigofera. J. Biol. Chem. 1963, 238: 1737–1742. 
  • Shiio I, Shiio T and McFadden BA. Isocitrate lyase from Pseudomonas indigofera. I. Preparation, amino acid composition and molecular weight. Biochim. Biophys. Acta. 1965, 96: 114–22. PMID 14285253. doi:10.1016/0005-2787(65)90615-5. 
  • VICKERY HB. A suggested new nomenclature for the isomers of isocitric acid. J. Biol. Chem. 1962, 237: 1739–41. PMID 13925783.