異檸檬酸裂合酶
有關縮寫為ICL的其他條目，請參見此
| Isocitrate Lyase | |||||||
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| Homotetrameric structure of Isocitrate lyase from E. coli. Based on PDB 1IGW.[1] | |||||||
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| 識別碼 | |||||||
| EC編號 | 4.1.3.1 | ||||||
| CAS號 | 9045-78-7 | ||||||
| 數據庫 | |||||||
| IntEnz | IntEnz瀏覽 | ||||||
| BRENDA | BRENDA入口 | ||||||
| ExPASy | NiceZyme瀏覽 | ||||||
| KEGG | KEGG入口 | ||||||
| MetaCyc | 代謝路徑 | ||||||
| PRIAM | 概述 | ||||||
| PDB | RCSB PDB PDBj PDBe PDBsum | ||||||
| 基因本體 | AmiGO / EGO | ||||||
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| Isocitrate lyase family | |||||||||
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| 鑑定 | |||||||||
| 標誌 | ICL | ||||||||
| Pfam | PF00463（舊版） | ||||||||
| InterPro | IPR000918 | ||||||||
| PROSITE | PDOC00145 | ||||||||
| SCOP | 1f8m / SUPFAM | ||||||||
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異檸檬酸裂合酶（英語：Isocitrate lyase，縮寫ICL，EC 4.1.3.1）是以一種在乙醛酸循環中將異檸檬酸切割為乙醛酸和琥珀酸的酶[2][3]。其產物再通過蘋果酸合酶合成蘋果酸，跳過了三羧酸循環（TCA循環）中脫去CO2的兩步。這一途徑廣泛存在於細菌、真菌與植物中。[4]
機制
[編輯]異檸檬酸裂合酶屬於異檸檬酸裂合酶家族，該家族的其它成員有羧乙烯基-羧基膦酸酯磷酰基變位酶（EC 2.7.8.23），以及磷酸烯醇式丙酮酸變位酶（EC 5.4.2.9，參與草丁膦類三肽抗生素的合成）。
在乙醛酸循環中，蘋果酸合酶可催化乙酰輔酶A與乙醛酸縮合形成蘋果酸從而使循環得以繼續。
酶結構
[編輯]截止2007年底，已有5個這類酶的結構被解析，PDB接入代碼為1DQU、1F61、1F8I、1F8M和1IGW。
另見
[編輯]參考文獻
[編輯]- ^ Britton, KL; Abeysinghe IS, Baker PJ, Barynin V, Diehl P, Langridge SJ, McFadden BA, Sedelnikova SE, Stillman TJ, Weeradechapon K, Rice DW. The structure and domain organization of Escherichia coli isocitrate lyase. Acta Crystallogr D. Sep 2001, 57 (9): 1209–1218. PMID 11526312. doi:10.1107/S0907444901008642.
- ^ Beeching JR. High sequence conservation between isocitrate lyase from Escherichia coli and Ricinus communis. Protein Seq. Data Anal. 1989, 2 (6): 463–466. PMID 2696959.
- ^ Tanaka A, Atomi H, Ueda M, Hikida M, Hishida T, Teranishi Y. Peroxisomal isocitrate lyase of the n-alkane-assimilating yeast Candida tropicalis: gene analysis and characterization. J. Biochem. 1990, 107 (2): 262–266. PMID 2361956.
- ^ Dunn, MF; Ramirez-Trujill JA; Hernandez-Lucas I. Major roles of isocitrate lyase and malate synthase in bacterial and fungal pathogenesis. Microbiology. Oct 2009, 155 (10): 3166–3175. PMID 19684068. doi:10.1099/mic.0.030858-0.
延伸閱讀
[編輯]- McFadden BA and Howes WV. Crystallisation and some properties of isocitrate lyase from Pseudomonas indigofera. J. Biol. Chem. 1963, 238: 1737–1742.
- Shiio I, Shiio T and McFadden BA. Isocitrate lyase from Pseudomonas indigofera. I. Preparation, amino acid composition and molecular weight. Biochim. Biophys. Acta. 1965, 96: 114–22. PMID 14285253. doi:10.1016/0005-2787(65)90615-5.
- VICKERY HB. A suggested new nomenclature for the isomers of isocitric acid. J. Biol. Chem. 1962, 237: 1739–41. PMID 13925783.