CTCF

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CCCTC-结合因子(锌指蛋白)

PDB rendering based on 1x6h.
有效结构
PDB 直系同源检索:PDBe, RCSB
标识
代号 CTCF
扩展标识 遗传学604167 鼠基因109447 同源基因4786 GeneCards: CTCF Gene
RNA表达模式
PBB GE CTCF 202521 at tn.png
更多表达数据
直系同源体
物种 人类 鼠类
Entrez 10664 13018
Ensembl ENSG00000102974 ENSMUSG00000005698
UniProt P49711 Q61164
mRNA序列 NM_001191022 NM_181322
蛋白序列 NP_001177951 NP_851839
基因位置 Chr 16:
67.6 – 67.67 Mb
Chr 8:
105.64 – 105.68 Mb
PubMed查询 [1] [2]

转录阻抑物CTCF(又叫11-锌指蛋白CCCTC-结合因子),是CTCF基因编码的转录因子[1][2],与绝缘子的活性相关。

功能[编辑]

CTCF蛋白在通过与H-19印记控制区域(imprinting control region,ICR)和分化甲基化区域1(differentially-methylated region-1,DMR1)和MAR3结合 抑制胰岛素样生长因子2基因的过程中起重要作用。[3][4] CTCF与靶顺序因子的结合可阻断增强子和启动子的相互作用。从而将增强子的活性限制在一定的功能区域。除了阻断增强子外,CTCF还可作为染色质屏障阻止异染色质的传播。 人类基因组有将近一万五千个CTCF绝缘体位点,说明CTCF在基因调控方面的功能广泛。[5][6] 而且CTCF结合位点还可作为核小体定位锚。[7]

相互作用[编辑]

CTCF可以结合另一个CTCF形成同源二聚体[8]。This activity is one possibility of the mechanism of its looping activity.

CTCF也和Y盒结合蛋白1英语Y box binding protein 1存在交互作用[9]。CTCF also co-localizes with cohesin, which stabilizes the repressive loops organized by the CTCF.[10]

参考[编辑]

  1. ^ Filippova GN, Fagerlie S, Klenova EM, Myers C, Dehner Y, Goodwin G, Neiman PE, Collins SJ, Lobanenkov VV. An exceptionally conserved transcriptional repressor, CTCF, employs different combinations of zinc fingers to bind diverged promoter sequences of avian and mammalian c-myc oncogenes. Mol. Cell. Biol. 1996.June, 16 (6): 2802–13. PMC 231272. PMID 8649389. 
  2. ^ Rubio ED, Reiss DJ, Welcsh PL, Disteche CM, Filippova GN, Baliga NS, Aebersold R, Ranish JA, Krumm A. CTCF physically links cohesin to chromatin. Proc. Natl. Acad. Sci. U.S.A. 2008.June, 105 (24): 8309–14. doi:10.1073/pnas.0801273105. PMC 2448833. PMID 18550811. 
  3. ^ Ohlsson R, Renkawitz R, Lobanenkov V. CTCF is a uniquely versatile transcription regulator linked to epigenetics and disease. Trends Genet. 2001, 17 (9): 520–7. doi:10.1016/S0168-9525(01)02366-6. PMID 11525835. 
  4. ^ Dunn KL, Davie JR. The many roles of the transcriptional regulator CTCF. Biochem. Cell Biol. 2003, 81 (3): 161–7. doi:10.1139/o03-052. PMID 12897849. 
  5. ^ Xie X, Mikkelsen TS, Gnirke A, Lindblad-Toh K, Kellis M, Lander ES. Systematic discovery of regulatory motifs in conserved regions of the human genome, including thousands of CTCF insulator sites. Proc. Natl. Acad. Sci. U.S.A. 2007, 104 (17): 7145–50. doi:10.1073/pnas.0701811104. PMC 1852749. PMID 17442748. 
  6. ^ Kim TH, Abdullaev ZK, Smith AD, Ching KA, Loukinov DI, Green RD, Zhang MQ, Lobanenkov VV, Ren B. Analysis of the vertebrate insulator protein CTCF-binding sites in the human genome. Cell. 2007, 128 (6): 1231–45. doi:10.1016/j.cell.2006.12.048. PMC 2572726. PMID 17382889. 
  7. ^ Fu Y, Sinha M, Peterson CL, Weng Z. The insulator binding protein CTCF positions 20 nucleosomes around its binding sites across the human genome. (编) Van Steensel, Bas. PLoS genetics. 2008, 4 (7): e1000138. doi:10.1371/journal.pgen.1000138. PMC 2453330. PMID 18654629. 
  8. ^ 引用错误:无效<ref>标签;未为name属性为Yusufzai_2004的引用提供文字
  9. ^ Chernukhin IV, Shamsuddin S, Robinson AF, Carne AF, Paul A, El-Kady AI, Lobanenkov VV, Klenova EM. Physical and functional interaction between two pluripotent proteins, the Y-box DNA/RNA-binding factor, YB-1, and the multivalent zinc finger factor, CTCF. J. Biol. Chem. 2000.September, 275 (38): 29915–21. doi:10.1074/jbc.M001538200. PMID 10906122. 
  10. ^ Kagey MH, Newman JJ, Bilodeau S, Zhan Y, Orlando DA, van Berkum NL, Ebmeier CC, Goossens J, Rahl PB, Levine SS, Taatjes DJ, Dekker J, Young RA. Mediator and cohesin connect gene expression and chromatin architecture. Nature. 2010.September, 467 (7314): 430–5. doi:10.1038/nature09380. PMC 2953795. PMID 20720539. 

衍生阅读[编辑]